Mad2L1 Recombinant Rabbit Monoclonal Antibody [JU46-13]
cat.: ET7106-69
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human
Applications: WB
Clonality: Monoclonal
Clone number: JU46-13
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 24 kDa
Isotype: IgG
Immunogen: Recombinant protein within Human Mad2L1 aa 69-124 / 205.
Positive control: K-562 cell lysates.
Subcellular location: Nucleus. Cytoskeleton.
Recommended Dilutions:
  WB

1:500-1:2,000
Uniprot #: SwissProt: Q13257 Human
Alternative names: Caught by MAD2 protein CMT2 dJ261G23.1 KIAA0110 MAD2L1 binding protein MAD2L1 binding protein isoform 2 MAD2L1-binding protein Mad2l1bp MD2BP_HUMAN MGC11282 OTTHUMP00000016496 RP1 261G23.6
Images
ET7106-69_1.jpg Fig1: Western blot analysis of Mad2L1 on K-562 cell lysates with Rabbit anti-Mad2L1 antibody (ET7106-69) at 1/1,000 dilution.

Lysates/proteins at 20 µg/Lane.

Predicted band size: 24 kDa
Observed band size: 25 kDa

Exposure time: 1 minute 2 seconds; ECL: K1801;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7106-69) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.