| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | JU46-13 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 24 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human Mad2L1 aa 69-124 / 205. |
| Positive control: | K-562 cell lysates. |
| Subcellular location: | Nucleus. Cytoskeleton. |
| Recommended Dilutions:
WB |
1:500-1:2,000 |
| Uniprot #: | SwissProt: Q13257 Human |
| Alternative names: | Caught by MAD2 protein CMT2 dJ261G23.1 KIAA0110 MAD2L1 binding protein MAD2L1 binding protein isoform 2 MAD2L1-binding protein Mad2l1bp MD2BP_HUMAN MGC11282 OTTHUMP00000016496 RP1 261G23.6 |
|
Fig1:
Western blot analysis of Mad2L1 on K-562 cell lysates with Rabbit anti-Mad2L1 antibody (ET7106-69) at 1/1,000 dilution. Lysates/proteins at 20 µg/Lane. Predicted band size: 24 kDa Observed band size: 25 kDa Exposure time: 1 minute 2 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7106-69) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |