GAB1 Recombinant Rabbit Monoclonal Antibody [JU47-19]
cat.: ET7106-70
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, IF-Tissue, IHC-P, FC |
| Clonality: | Monoclonal |
| Clone number: | JU47-19 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | 77 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human GAB1 aa 450-500 . |
| Positive control: | Human small intestine tissue lysate, LOVO, MCF-7, SH-SY5Y, human skin tissue, rat epididymis tissue, Hela. |
| Subcellular location: | Cytosol. |
| Recommended Dilutions:
WB IF-Cell IHC-P FC |
1:500-1:2,000 1:100-1:500 1:50-1:200 1:50-1:100 |
| Uniprot #: | SwissProt: Q13480 Human |
| Alternative names: | Gab 1 GAB1 GAB1_HUMAN GRB 2 associated binder 1 GRB 2 associated binding protein 1 GRB2 associated binding protein 1 isoform a GRB2 associated binding protein 1 isoform b GRB2-associated binder 1 GRB2-associated-binding protein 1 Growth factor receptor bound protein 2-associated protein 1 |
Images
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Fig1: Western blot analysis of GAB1 on human small intestine tissue lysate using anti-GAB1 antibody at 1/500 dilution. |
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Fig2: ICC staining GAB1 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig3: ICC staining GAB1 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig4: ICC staining GAB1 in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig5: Immunohistochemical analysis of paraffin-embedded human skin tissue using anti-GAB1 antibody. Counter stained with hematoxylin. |
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Fig6: Immunohistochemical analysis of paraffin-embedded rat epididymis tissue using anti-GAB1 antibody. Counter stained with hematoxylin. |
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Fig7: Flow cytometric analysis of Hela cells with GAB1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.