GAB1 Recombinant Rabbit Monoclonal Antibody [JU47-19]
cat.: ET7106-70
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IF-Cell, IF-Tissue, IHC-P, FC
Clonality: Monoclonal
Clone number: JU47-19
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: 77 kDa
Isotype: IgG
Immunogen: Synthetic peptide within Human GAB1 aa 450-500 .
Positive control: Human small intestine tissue lysate, LOVO, MCF-7, SH-SY5Y, human skin tissue, rat epididymis tissue, Hela.
Subcellular location: Cytosol.
Recommended Dilutions:
  WB
  IF-Cell
  IHC-P
  FC

1:500-1:2,000
1:100-1:500
1:50-1:200
1:50-1:100
Uniprot #: SwissProt: Q13480 Human
Alternative names: Gab 1 GAB1 GAB1_HUMAN GRB 2 associated binder 1 GRB 2 associated binding protein 1 GRB2 associated binding protein 1 isoform a GRB2 associated binding protein 1 isoform b GRB2-associated binder 1 GRB2-associated-binding protein 1 Growth factor receptor bound protein 2-associated protein 1
Images
ET7106-70_1.jpg Fig1: Western blot analysis of GAB1 on human small intestine tissue lysate using anti-GAB1 antibody at 1/500 dilution.
ET7106-70_2.jpg Fig2: ICC staining GAB1 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ET7106-70_3.jpg Fig3: ICC staining GAB1 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ET7106-70_4.jpg Fig4: ICC staining GAB1 in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ET7106-70_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded human skin tissue using anti-GAB1 antibody. Counter stained with hematoxylin.
ET7106-70_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded rat epididymis tissue using anti-GAB1 antibody. Counter stained with hematoxylin.
ET7106-70_7.jpg Fig7: Flow cytometric analysis of Hela cells with GAB1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.