SCGB1A1 Recombinant Rabbit Monoclonal Antibody [JU34-03]
cat.: ET7106-71
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, FC |
| Clonality: | Monoclonal |
| Clone number: | JU34-03 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 10 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human SCGB1A1 aa 42-91 / 91. |
| Positive control: | MCF7 cell lysate, NIH/3T3 cell lysate, C6 cell lysate, A431, A549, HUVEC. |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
WB IF-Cell FC |
1:500-1:1,000 1:50-1:200 1:50-1:100 |
| Uniprot #: | SwissProt: P11684 Human | Q06318 Mouse | P17559 Rat |
| Alternative names: | Blastokinin CC10 CC16 CCPBP CCSP Clara cell phospholipid binding protein Clara cell phospholipid-binding protein Clara cell specific 10 kD protein Clara cells 10 kDa secretory protein OTTHUMP00000236107 SCGB1A1 Secretoglobin family 1A member 1 Secretoglobin, family 1A, member 1 (uteroglobin) UG UGB UP-1 UP1 Urinary protein 1 Urine protein 1 UTER_HUMAN Uteroglobin |
Images
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Fig1:
Western blot analysis of SCGB1A1 on different lysates with Rabbit anti-SCGB1A1 antibody (ET7106-71) at 1/1,000 dilution. Lane 1: MCF7 (Human breast cancer cell) cell lysate Lane 2: NIH/3T3 (Mouse fibroblast) cell lysate Lane 3: C6 (Rat glioma cell) cell lysate Lysates/proteins at 10 µg/Lane. Exposure time: 180 seconds; ECL: K1802 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: ET7106-71, 1/1,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 10 kDa Observed band size: 15 kDa |
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Fig2: ICC staining of SCGB1A1 in A431 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET7106-71, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue). |
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Fig3: ICC staining of SCGB1A1 in A549 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET7106-71, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue). |
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Fig4: ICC staining of SCGB1A1 in HUVEC cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET7106-71, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue). |
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Fig5: Flow cytometric analysis of SCGB1A1 was done on A549 cells. The cells were fixed, permeabilized and stained with the primary antibody (ET7106-71, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.