ADAR Recombinant Rabbit Monoclonal Antibody [JU99-33]
cat.: ET7106-72
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human
Applications: WB, IF-Cell, IF-Tissue, IHC-P
Clonality: Monoclonal
Clone number: JU99-33
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 136 kDa
Isotype: IgG
Immunogen: Recombinant protein within Human ADAR aa 180-280 / 1,226.
Positive control: SiHa cell lysates, Hela, human colon carcinoma tissue, human kidney tissue, human lung carcinoma tissue, human pancreas tissue.
Subcellular location: Cytoplasm. Nucleus.
Recommended Dilutions:
  WB
  IF-Cell
  IHC-P
1:500-1:2,000
1:50-1:200
1:50-1:200
Uniprot #: SwissProt: P55265 Human
Alternative names: 136 kDa double-stranded RNA-binding protein 136kDa double stranded RNA binding protein Adar 1 ADAR Adar1 Adenosine deaminase acting on RNA 1 A Adenosine deaminase RNA specific 1 Adenosine deaminase RNA specific Adenosine deaminase that act on RNA AGS6 AV242451 Double stranded RNA specific adenosine deaminase Double-stranded RNA-specific adenosine deaminase Double-stranded RNA-specific editase Adar DRADA Dsh Dsrad DSRAD_HUMAN dsRNA adenosine deaminase EC 3.5.4.- G1P1 IFI 4 IFI-4 IFI4 Ifi4 protein Interferon induced protein 4 Interferon inducible protein 4 Interferon-inducible protein 4 K88DSRBP mZaADAR P136 Pre-mRNA adenosine deaminase RNA adenosine deaminase 1 RNA-editing deaminase 1 RNA-editing enzyme 1
Images
ET7106-72_1.jpg Fig1: Western blot analysis of ADAR on SiHa cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST in PBS for 1 hour at room temperature. The primary antibody (ET7106-72, 1/500) was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.

Predicted band size: 136 kDa
Observed band size: 136 kDa
ET7106-72_2.jpg Fig2: ICC staining of ADAR in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (ET7106-72, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ET7106-72_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using anti-ADAR antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7106-72, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET7106-72_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-ADAR antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7106-72, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET7106-72_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using anti-ADAR antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7106-72, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET7106-72_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-ADAR antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7106-72, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.