PIWIL1 Recombinant Rabbit Monoclonal Antibody [JU35-12]
cat.: ET7106-73
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JU35-12 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 99 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human PIWIL1 aa 733-831 . |
| Positive control: | Mouse testis tissue lysates, rat testis tissue, mouse testis tissue. |
| Subcellular location: | Cytoplasm. |
| Recommended Dilutions:
WB IHC-P |
1:500-1:1,000 1:50-1:200 |
| Uniprot #: | SwissProt: Q96J94 Human | Q9JMB7 Mouse Entrez Gene: 363912 Rat |
| Alternative names: | HIWI MIWI Piwi (Drosophila) like 1 PIWI Piwi homolog Piwi like 1 (drosophila) Piwi like 1 Piwi-like protein 1 PIWIL1 PIWL1_HUMAN |
Images
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Fig1: Western blot analysis of PIWIL1 on mouse testis tissue lysate using anti-PIWIL1 antibody at 1/500 dilution. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded rat testis tissue with Rabbit anti-PIWIL1 antibody (ET7106-73) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7106-73) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig3:
Immunohistochemical analysis of paraffin-embedded mouse testis tissue with Rabbit anti-PIWIL1 antibody (ET7106-73) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7106-73) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.