BDKRB2 Recombinant Rabbit Monoclonal Antibody [JU39-04]
cat.: ET7106-74
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB, FC, IP, IHC-P, IF-Cell |
| Clonality: | Monoclonal |
| Clone number: | JU39-04 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 44 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human BDKRB2 aa 262-391 / 391. |
| Positive control: | Neuro-2a cell lysates, MCF-7 cell lysates, mouse brain tissue, Neuro-2a, SH-SY5Y. |
| Subcellular location: | Cell membrane. |
| Recommended Dilutions:
WB FC IP IHC-P IF-Cell |
1:500-1:2,000 1:1:1,000 1-2μg/sample 1:200 1:100 |
| Uniprot #: | SwissProt: P30411 Human | P32299 Mouse |
| Alternative names: | B2 B2 bradykinin receptor B2BKR B2BRA B2R BDKR B2 BDKRB 2 BDKRB2 BK 2 BK 2 receptor BK R2 BK-2 receptor BK2 BK2 receptor BK2R BKR 2 BKR2 BKRB2_HUMAN BR B2 Bradykinin receptor B2 Bradykinin receptor beta 2 BRB 2 BRB2 DKFZp686O088 Kinin B2 |
Images
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Fig1:
Western blot analysis of BDKRB2 on Neuro-2a cell lysates with Rabbit anti-BDKRB2 antibody (ET7106-74) at 1/2,000 dilution. Lysates/proteins at 20 µg/Lane. Predicted band size: 44 kDa Observed band size: 80-90 kDa Exposure time: 20 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7106-74) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2: Western blot analysis of BDKRB2 on MCF-7 cell lysate using anti-BDKRB2 antibody at 1/500 dilution. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-BDKRB2 antibody (ET7106-74) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7106-74) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Immunocytochemistry analysis of Neuro-2a cells labeling BDKRB2 with Rabbit anti-BDKRB2 antibody (ET7106-74) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-BDKRB2 antibody (ET7106-74) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
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Fig5:
Flow cytometric analysis of Neuro-2a cells labeling BDKRB2. Cells were fixed and permeabilized. Then stained with the primary antibody (ET7106-74, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
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Fig6:
BDKRB2 was immunoprecipitated from 0.2 mg Neuro-2a cell lysate with ET7106-74 at 2 µg/25 µl agarose. Western blot was performed from the immunoprecipitate using ET7106-74 at 1/2,000 dilution. Mouse Anti-Rabbit IgG kappa light chain secondary antibody (M1208-2) at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: Neuro-2a cell lysate (input) Lane 2: ET7106-74 IP in Neuro-2a cell lysate Lane 3: Rabbit IgG instead of ET7106-74 in Neuro-2a cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 2 seconds; ECL: K1801 |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.