SMURF 2 Recombinant Rabbit Monoclonal Antibody [JU32-34]
cat.: ET7106-76
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human
Applications: WB
Clonality: Monoclonal
Clone number: JU32-34
Form: Liquid
Storage condition: Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: 86 kDa
Isotype: IgG
Immunogen: Recombinant protein within Human SMURF 2 aa 150-340.
Positive control: A431.
Subcellular location: Cytoplasm. Membrane. Nucleus.
Recommended Dilutions:
  WB

1:500
Uniprot #: SwissProt: Q9HAU4 Human
Alternative names: E3 ubiquitin-protein ligase SMURF2 EC 6.3.2. hSMURF2 MGC138150 Smad specific E3 ubiquitin ligase 2 SMAD specific E3 ubiquitin protein ligase 2 SMAD ubiquitination regulatory factor 2 SMAD-specific E3 ubiquitin-protein ligase 2 SMUF2_HUMAN Smurf2 Ubiquitin protein ligase SMURF2
Images
ET7106-76_1.jpg Fig1: Western blot analysis of SMURF 2 on Rrat testis tissue lysates with Rabbit anti-SMURF 2 antibody (ET7106-76) at 1/1,000 dilution.

Lysates/proteins at 15 µg/Lane.
Exposure time: 60 seconds; ECL: K1801

Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: ET7106-76, 1/1,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature

Predicted band size: 86 kDa
Observed band size: 86 kDa
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.