Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
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Species reactivity: | Human |
Applications: | WB, IF-Cell |
Clonality: | Monoclonal |
Clone number: | JU31-30 |
Form: | Liquid |
Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
Concentration: | 1ug/ul |
Purification: | Protein A affinity purified. |
Molecular weight: | 36 kDa |
Isotype: | IgG |
Immunogen: | Synthetic peptide within Human Endo G aa 200-297 / 297. |
Positive control: | MCF-7, PC-3M, SH-SY5Y, mouse heart tissue lysate. |
Subcellular location: | Mitochondrion. Nucleus. |
Recommended Dilutions:
WB IF-Cell |
1:500-1:1,000 1:50-1:200 |
Uniprot #: | SwissProt: Q14249 Human |
Alternative names: | EndoG Endonuclease G Endonuclease G mitochondrial EndonucleaseG FLJ27463 Mitochondrial endonuclease G NUCG_HUMAN |
Fig1: Western blot analysis of Endo G on mouse heart tissue lysate using anti-Endo G antibody at 1/500 dilution. | |
Fig2: ICC staining Endo G in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. | |
Fig3: ICC staining Endo G in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. | |
Fig4: ICC staining Endo G in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised |