| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB, IF-Cell |
| Clonality: | Monoclonal |
| Clone number: | JU31-30 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | 36 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human Endo G aa 200-297 / 297. |
| Positive control: | MCF-7, PC-3M, SH-SY5Y, mouse heart tissue lysate. |
| Subcellular location: | Mitochondrion. Nucleus. |
| Recommended Dilutions:
WB IF-Cell |
1:500-1:1,000 1:50-1:200 |
| Uniprot #: | SwissProt: Q14249 Human | O08600 Mouse |
| Alternative names: | EndoG Endonuclease G Endonuclease G mitochondrial EndonucleaseG FLJ27463 Mitochondrial endonuclease G NUCG_HUMAN |
|
Fig1: Western blot analysis of Endo G on mouse heart tissue lysate using anti-Endo G antibody at 1/500 dilution. |
|
Fig2: ICC staining Endo G in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
|
Fig3: ICC staining Endo G in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
|
Fig4: ICC staining Endo G in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised |