Endo G Recombinant Rabbit Monoclonal Antibody [JU31-30]
cat.: ET7106-77
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB, IF-Cell |
| Clonality: | Monoclonal |
| Clone number: | JU31-30 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | 36 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human Endo G aa 200-297 / 297. |
| Positive control: | MCF-7, PC-3M, SH-SY5Y, mouse heart tissue lysate. |
| Subcellular location: | Mitochondrion. Nucleus. |
| Recommended Dilutions:
WB IF-Cell |
1:500-1:1,000 1:50-1:200 |
| Uniprot #: | SwissProt: Q14249 Human | O08600 Mouse |
| Alternative names: | EndoG Endonuclease G Endonuclease G mitochondrial EndonucleaseG FLJ27463 Mitochondrial endonuclease G NUCG_HUMAN |
Images
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Fig1: ICC staining Endo G in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig2: ICC staining Endo G in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig3: ICC staining Endo G in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised |
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Fig4:
Western blot analysis of Endo G on different lysates with Rabbit anti-Endo G antibody (ET7106-77) at 1/1,000 dilution. Lane 1: Mouse heart tissue lysate Exposure time: 3 minutes 10 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: ET7106-77, 1/1,000 in 5% NFDM/TBST, 2 hour at room temperature Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 32.6 kDa Observed band size: 36 kDa |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.