Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
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Species reactivity: | Human, Mouse |
Applications: | WB, IF-Cell, IHC-P, FC |
Clonality: | Monoclonal |
Clone number: | JU37-48 |
Form: | Liquid |
Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
Concentration: | 1ug/ul |
Purification: | Protein A affinity purified. |
Molecular weight: | 86/63 kDa |
Isotype: | IgG |
Immunogen: | Recombinant protein within Human SEC23 aa 280-420 / 765. |
Positive control: | NIH-3T3, HepG2, HUVEC, human tonsil tissue, human fetal skeletal muscle tissue, HepG2. |
Subcellular location: | Cytosol. Endoplasmic reticulum. |
Recommended Dilutions:
WB IF-Cell IHC-P FC |
1:500-1:1,000 1:50-1:200 1:50-1:200 1:50-1:100 |
Uniprot #: | SwissProt: Q15436 Human | Q01405 Mouse |
Alternative names: | CLSD Protein transport protein Sec23A SC23A_HUMAN Sec23 homolog A (S. cerevisiae) SEC23-related protein A sec23a |
Fig1: Western blot analysis of SEC23 on NIH-3T3 cell using anti-SEC23 antibody at 1/500 dilution. | |
Fig2: ICC staining SEC23 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. | |
Fig3: ICC staining SEC23 in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. | |
Fig4: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-SEC23 antibody. Counter stained with hematoxylin. |
Fig5: Immunohistochemical analysis of paraffin-embedded human fetal skeletal muscle tissue using anti-SEC23 antibody. Counter stained with hematoxylin. | |
Fig6: Flow cytometric analysis of HepG2 cells with SEC23 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Goat anti rabbit IgG (FITC) was used as the secondary antibody. |