PCAF Recombinant Rabbit Monoclonal Antibody [JU88-63]
cat.: ET7106-79
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IP, FC |
| Clonality: | Monoclonal |
| Clone number: | JU88-63 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 93 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human PCAF aa 121-211 . |
| Positive control: | HeLa cell lysates, Hela. |
| Subcellular location: | Nucleus. |
| Recommended Dilutions:
WB FC IP |
1:2,000 1:50-1:100 1:10-1:50 |
| Uniprot #: | SwissProt: Q92831 Human | Q568Z2 Rat |
| Alternative names: | CAF CREBBP associated factor GCN5 GCN5L GCN5L1 Histone acetylase PCAF Histone acetyltransferase KAT2B Histone acetyltransferase PCAF K(lysine) acetyltransferase 2B KAT2B KAT2B_HUMAN Lysine acetyltransferase 2B P P/CAF p300/CBP associated factor P300/CBP-associated factor Pcaf |
Images
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Fig1:
Western blot analysis of PCAF on HeLa cell lysates with Rabbit anti-PCAF antibody (ET7106-79) at 1/2,000 dilution. Lysates/proteins at 20 µg/Lane. Predicted band size: 93 kDa Observed band size: 93 kDa Exposure time: 5 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7106-79) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2: Flow cytometric analysis of Hela cells with PCAF antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.