PCK1 Recombinant Rabbit Monoclonal Antibody [JU84-39]
cat.: ET7106-81
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | IF-Cell, IHC-P, WB |
| Clonality: | Monoclonal |
| Clone number: | JU84-39 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | 69 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human PCK1 aa 26-75 / 622. |
| Positive control: | 293T, HepG2, SH-SY5Y, human liver tissue, human kidney tissue. |
| Subcellular location: | Cytoplasm. |
| Recommended Dilutions:
IF-Cell IHC-P WB |
1:50-1:100 1:200 1:500 |
| Uniprot #: | SwissProt: P35558 Human |
| Alternative names: | cytosolic [GTP] GTP PCK1 PCKGC_HUMAN PEP carboxykinase PEPCK-C PEPCK1 PEPCKC Phosphoenolpyruvate carboxykinase 1 (soluble) Phosphoenolpyruvate carboxykinase 1 Phosphoenolpyruvate carboxykinase Phosphoenolpyruvate carboxykinase, cytosolic [GTP] Phosphoenolpyruvate carboxykinase, cytosolic Phosphoenolpyruvate carboxylase Phosphopyruvate carboxylase |
Images
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Fig1:
Immunocytochemistry analysis of 293T cells labeling PCK1 with Rabbit anti-PCK1 antibody (ET7106-81) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-PCK1 antibody (ET7106-81) at 1/100 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. |
|
Fig2:
Immunocytochemistry analysis of HepG2 cells labeling PCK1 with Rabbit anti-PCK1 antibody (ET7106-81) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-PCK1 antibody (ET7106-81) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. |
|
Fig3:
Immunocytochemistry analysis of SH-SY5Y cells labeling PCK1 with Rabbit anti-PCK1 antibody (ET7106-81) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-PCK1 antibody (ET7106-81) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. |
|
Fig4:
Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-PCK1 antibody (ET7106-81) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7106-81) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5:
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-PCK1 antibody (ET7106-81) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7106-81) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.