MAPKAP Kinase 2 Recombinant Rabbit Monoclonal Antibody [JU30-31]
cat.: ET7106-83
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB, IP |
| Clonality: | Monoclonal |
| Clone number: | JU30-31 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 46 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human MAPKAP Kinase 2 aa 351-400 / 400. |
| Positive control: | SK-Br-3 cell lysates. |
| Subcellular location: | Cytoplasm. Nucleus. |
| Recommended Dilutions:
WB IP |
1:500-1:1,000 1:10-1:50 |
| Uniprot #: | SwissProt: P49137 Human | P49138 Mouse |
| Alternative names: | MAP kinase activated protein Kinase 2 MAP kinase-activated protein kinase 2 MAPK activated protein kinase 2 MAPK-activated protein kinase 2 MAPK2_HUMAN MAPKAP K2 MAPKAP kinase 2 MAPKAPK 2 MAPKAPK-2 MAPKAPK2 Mitogen activated protein kinase activated protein kinase 2 MK 2 MK2 |
Images
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Fig1:
Western blot analysis of MAPKAP Kinase 2 on different lysates with Rabbit anti-MAPKAP Kinase 2 antibody (ET7106-83) at 1/2,000 dilution. Lane 1: HCT 116-si NT cell lysate Lane 2: HCT 116-si MAPKAP Kinase 2 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 46 kDa Observed band size: 46 kDa Exposure time: 8 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7106-83) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Western blot analysis of MAPKAP Kinase 2 on SK-Br-3 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET7106-83, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. Predicted band size: 46 kDa Observed band size: 46 kDa |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.