MMP17 Recombinant Rabbit Monoclonal Antibody [JU33-03]
cat.: ET7106-84
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | IF-Cell, IF-Tissue, IHC-P, WB |
| Clonality: | Monoclonal |
| Clone number: | JU33-03 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | 67 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human MMP17 aa 175-225 / 603. |
| Positive control: | A549, MCF-7, mouse brain tissue. |
| Subcellular location: | Secreted. Plasma membrane. |
| Recommended Dilutions:
IF-Cell IHC-P WB |
1:50 1:50 1:500 |
| Uniprot #: | SwissProt: Q9ULZ9 Human | Q9R0S3 Mouse |
| Alternative names: | Matrix metalloproteinase 17 Matrix metalloproteinase 17 membrane inserted Matrix metalloproteinase-17 Membrane type 4 matrix metalloproteinase Membrane type matrix metalloproteinase 4 Membrane-type matrix metalloproteinase 4 Membrane-type-4 matrix metalloproteinase MMP 17 MMP-17 Mmp17 MMP17_HUMAN MT MMP 4 MT MMP4 MT-MMP 4 MT4 MMP MT4-MMP MT4MMP MTMMP 4 MTMMP4 |
Images
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Fig1: ICC staining of MMP17 in A549 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (ET7106-84, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue). |
|
Fig2: ICC staining of MMP17 in MCF-7 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (ET7106-84, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue). |
|
Fig3: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-MMP17 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7106-84, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.