NR0B1 Recombinant Rabbit Monoclonal Antibody [JU33-05]
cat.: ET7106-85
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IF-Cell, FC |
| Clonality: | Monoclonal |
| Clone number: | JU33-05 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | 52 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human NR0B1 aa 1-50 / 470. |
| Positive control: | A549, Hela, SH-SY-5Y. |
| Subcellular location: | Cytoplasm. Nucleus. |
| Recommended Dilutions:
WB IF-Cell FC |
1:500 1:50-1:200 1:50-1:100 |
| Uniprot #: | SwissProt: P51843 Human |
| Alternative names: | AHC AHCH AHX DAX 1 DAX1 Dosage sensitive sex reversal DSS DSS AHC critical region on the X chromosome protein 1 DSS-AHC critical region on the X chromosome protein 1 GTD HHG Nr0b1 NR0B1_HUMAN NROB1 Nuclear hormone receptor Nuclear receptor 0B1 Nuclear receptor DAX 1 Nuclear receptor DAX-1 Nuclear receptor DAX1 Nuclear receptor subfamily 0 group B member 1 SRXY2 |
Images
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Fig1: Western blot analysis of NR0B1 on A549 cell lysate using anti-NR0B1 antibody at 1/500 dilution. |
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Fig2: ICC staining NR0B1 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig3: ICC staining NR0B1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig4: ICC staining NR0B1 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig5: Flow cytometric analysis of A549 cells with NR0B1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.