GABA B Receptor 2 Recombinant Rabbit Monoclonal Antibody [JU31-32]
cat.: ET7106-88
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat, Cynomolgus monkey, Pig |
| Applications: | WB, IHC-P, IHC-Fr, IF-Tissue |
| Clonality: | Monoclonal |
| Clone number: | JU31-32 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 106 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within C-terminal Human GABA B Receptor 2 . |
| Positive control: | Mouse brain tissue lysate, rat brain tissue lysate, mouse brain tissue, rat brain tissue, mouse cerebellum tissue, SH-SY-5Y. |
| Subcellular location: | Plasma membrane. |
| Recommended Dilutions:
WB IHC-P IHC-Fr IF-Tissue |
1:1,000 1:1,000 1:500-1:1,000 1:500 |
| Uniprot #: | SwissProt: O75899 Human | Q80T41 Mouse | O88871 Rat |
| Alternative names: | BcDNA:GH07312 CG6706 CT20836 D Gaba2 FLJ36928 G protein coupled receptor 51 G-protein coupled receptor 51 GAB B R2 GABA B R2 GABA B receptor 2 GABA-B receptor 2 GABA-B-R2 GABA-BR2 GABAB R2 GABABR 2 GABABR2 GABB R2 GABBR 2 Gabbr2 GABR2_HUMAN Gamma aminobutyric acid B receptor 2 Gamma aminobutyric acid GABA B receptor 2 Gamma aminobutyric acid type B receptor subunit 2 Gamma-aminobutyric acid type B receptor subunit 2 Gb 2 Gb2 GH07312 GPR 51 GPR51 GPRC 3B GPRC3B HG 20 HG20 HRIHFB2099 Metabotropic GABA B receptor subtype 2 OTTHUMP00000021776 OTTHUMP00000063797 R2 SUBUNIT |
Images
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Fig1:
Application: IHC-Fr Species: Mouse Site: Cerebellum Sample: Frozen section Antibody concentration: 1:1,000 Antigen retrieval: Not required |
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Fig2:
Application: IHC-Fr Species: Rat Site: Cerebellum Sample: Frozen section Antibody concentration: 1:1,000 Antigen retrieval: Not required |
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Fig3:
Application: IHC-Fr Species: Mouse Site: Cerebral cortex Sample: Frozen section Antibody concentration: 1:500 Antigen retrieval: Not required |
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Fig4:
Application: IF-tissue Species: Mouse Site: Cerebral cortex Sample: Paraffin-embedded section Antibody concentration: 1:500 |
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Fig5:
Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-GABA B Receptor 2 antibody (ET7106-88) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7106-88) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig6:
Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-GABA B Receptor 2 antibody (ET7106-88) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7106-88) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig7:
Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue with Rabbit anti-GABA B Receptor 2 antibody (ET7106-88) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7106-88) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig8:
Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue with Rabbit anti-GABA B Receptor 2 antibody (ET7106-88) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7106-88) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig9:
Western blot analysis of GABA B Receptor 2 on different lysates with Rabbit anti-GABA B Receptor 2 antibody (ET7106-88) at 1/1,000 dilution. Lane 1: Mouse brain tissue lysate Lane 2: Mouse brain tissue lysate (no heat) Lane 3: Rat brain tissue lysate Lane 4: Rat brain tissue lysate (no heat) Notice: no heat means the lysate is not boiled. Lysates/proteins at 20 µg/Lane. Predicted band size: 106 kDa Observed band size: 106 kDa Exposure time: 30 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7106-88) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.