GABA B Receptor 2 Recombinant Rabbit Monoclonal Antibody [JU31-32]
cat.: ET7106-88
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IHC-P, FC, IF-Cell, IHC-Fr, IF-Tissue
Clonality: Monoclonal
Clone number: JU31-32
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 106 kDa
Isotype: IgG
Immunogen: Recombinant protein within C-terminal Human GABA B Receptor 2 .
Positive control: Mouse brain tissue lysate, rat brain tissue lysate, mouse brain tissue, rat brain tissue, mouse cerebellum tissue, SH-SY-5Y.
Subcellular location: Plasma membrane.
Recommended Dilutions:
  WB
  IHC-P
  FC
  IF-Cell
  IHC-Fr
  IF-Tissue
1:1,000
1:50-1:200
1:50-1:100
1:50-1:100
1:500
1:500
Uniprot #: SwissProt: O75899 Human | Q80T41 Mouse | O88871 Rat
Alternative names: BcDNA:GH07312 CG6706 CT20836 D Gaba2 FLJ36928 G protein coupled receptor 51 G-protein coupled receptor 51 GAB B R2 GABA B R2 GABA B receptor 2 GABA-B receptor 2 GABA-B-R2 GABA-BR2 GABAB R2 GABABR 2 GABABR2 GABB R2 GABBR 2 Gabbr2 GABR2_HUMAN Gamma aminobutyric acid B receptor 2 Gamma aminobutyric acid GABA B receptor 2 Gamma aminobutyric acid type B receptor subunit 2 Gamma-aminobutyric acid type B receptor subunit 2 Gb 2 Gb2 GH07312 GPR 51 GPR51 GPRC 3B GPRC3B HG 20 HG20 HRIHFB2099 Metabotropic GABA B receptor subtype 2 OTTHUMP00000021776 OTTHUMP00000063797 R2 SUBUNIT
Images
ET7106-88_1.jpg Fig1: Immunofluorescence analysis of frozen mouse brain tissue with Rabbit anti-GABA B Receptor 2 antibody (ET7106-88) at 1/200 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for about 2 minutes in microwave oven. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (ET7106-88, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).
ET7106-88_2.jpg Fig2: Western blot analysis of GABA B Receptor 2 on different lysates with Rabbit anti-GABA B Receptor 2 antibody (ET7106-88) at 1/1,000 dilution.

Lane 1: Mouse brain tissue lysate (20 µg/Lane)
Lane 2: Mouse brain tissue lysate (no heat) (20 µg/Lane)
Lane 3: Rat brain tissue lysate (20 µg/Lane)
Lane 4: Rat brain tissue lysate (no heat) (20 µg/Lane)

Predicted band size: 106 kDa
Observed band size: 106 kDa

Exposure time: 30 seconds;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7106-88) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
ET7106-88_3.jpg Fig3: Immunofluorescence analysis of paraffin-embedded mouse brain tissue labeling GABA B Receptor 2 with Rabbit anti-GABA B Receptor 2 antibody (ET7106-88) at 1/50 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (ET7106-88, green) at 1/50 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).
ET7106-88_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-GABA B Receptor 2 antibody (ET7106-88) at 1/200 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7106-88) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET7106-88_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-GABA B Receptor 2 antibody (ET7106-88) at 1/200 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7106-88) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET7106-88_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue using anti- GABA B Receptor 2 antibody. Counter stained with hematoxylin.
ET7106-88_7.jpg Fig7: Flow cytometric analysis of SH-SY-5Y cells with GABA B Receptor 2 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.