Cellular Apoptosis Susceptibility Recombinant Rabbit Monoclonal Antibody [JU34-33]
cat.: ET7106-90
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IF-Cell, IF-Tissue, IHC-P, FC
Clonality: Monoclonal
Clone number: JU34-33
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 110 kDa
Isotype: IgG
Immunogen: Recombinant protein within Human Cellular Apoptosis Susceptibility aa 860-971 / 971.
Positive control: SW480 cell lysate, MCF7 cell lysate, SK-Br-3 cell lysate, SiHa cell lysate, PC-3M cell lysate, HeLa cell lysate, Ramos cell lysate, mouse testis tissue lysate, LOVO, PC-3M, SH-SY5Y, human colon carcinoma tissue, mouse testis tissue, LOVO.
Subcellular location: Cytoplasm. Nucleus.
Recommended Dilutions:
  WB
  IF-Cell
  IHC-P
  FC
1:1,000
1:50-1:200
1:200-1:1,000
1:50-1:100
Uniprot #: SwissProt: P55060 Human | Q9ERK4 Mouse
Alternative names: CAS Cellular apoptosis susceptibility protein Chromosome segregation 1 (yeast homolog) like Chromosome segregation 1 Like Chromosome segregation 1 like protein Chromosome segregation 1-like protein Chromosome segregation gene CSE1 CSE 1 CSE 1 chromosome segregation 1 like CSE 1 chromosome segregation 1 like protein CSE 1L CSE1 CSE1 chromosome segregation 1 like (yeast) CSE1 chromosome segregation 1 like CSE1 chromosome segregation 1 like protein CSE1L Exp 2 Exp2 Exportin 2 Exportin-2 Exportin2 Importin alpha re exporter Importin-alpha re-exporter MGC117283 MGC130036 MGC130037 XPO 2 XPO2 XPO2_HUMAN
Images
ET7106-90_1.jpg Fig1: Western blot analysis of Cellular Apoptosis Susceptibility on different lysates with Rabbit anti-Cellular Apoptosis Susceptibility antibody (ET7106-90) at 1/1,000 dilution.

Lane 1: SW480 cell lysate (20 µg/Lane)
Lane 2: MCF7 cell lysate (20 µg/Lane)
Lane 3: SK-Br-3 cell lysate (20 µg/Lane)
Lane 4: SiHa cell lysate (20 µg/Lane)
Lane 5: PC-3M cell lysate (20 µg/Lane)
Lane 6: HeLa cell lysate (20 µg/Lane)
Lane 7: Ramos cell lysate (20 µg/Lane)
Lane 8: Mouse testis tissue lysate (40 µg/Lane)

Predicted band size: 110 kDa
Observed band size: 110/50 kDa

Exposure time: 30 seconds;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7106-90) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.
ET7106-90_2.jpg Fig2: ICC staining Cellular Apoptosis Susceptibility in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ET7106-90_3.jpg Fig3: ICC staining Cellular Apoptosis Susceptibility in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ET7106-90_4.jpg Fig4: ICC staining Cellular Apoptosis Susceptibility in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ET7106-90_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue with Rabbit anti-Cellular Apoptosis Susceptibility antibody (ET7106-90) at 1/200 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7106-90) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET7106-90_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded mouse testis tissue with Rabbit anti-Cellular Apoptosis Susceptibility antibody (ET7106-90) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7106-90) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET7106-90_7.jpg Fig7: Flow cytometric analysis of LOVO cells with Cellular Apoptosis Susceptibility antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.