ET7106-91

GIT1 Recombinant Rabbit Monoclonal Antibody [JU33-39]

cat.: ET7106-91

Product Type:	Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity:	Human, Mouse, Rat
Applications:	WB, IHC-P, FC
Clonality:	Monoclonal
Clone number:	JU33-39

Form:	Liquid
Storage condition:	Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer:	1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration:	1ug/ul
Purification:	Protein A affinity purified.
Molecular weight:	Predicted band size: 84 kDa
Isotype:	IgG
Immunogen:	Recombinant protein within Human GIT1 aa 79-297 .
Positive control:	A549 cell lysates, human colon carcinoma tissue, human pancreas tissue, mouse fallopian tubes, rat brain tissue, A549, mouse small intestine tissue.
Subcellular location:	Cytoplasm.
Recommended Dilutions: WB IHC-P FC	1:500-1:1,000 1:50-1:400 1:50-1:100
Uniprot #:	SwissProt: Q9Y2X7 Human \| Q68FF6 Mouse \| Q9Z272 Rat
Alternative names:	ARF GAP GIT1 ARF GTPase activating protein GIT1 ARF GTPase-activating protein GIT1 CAT 1 CAT-1 CaT1 Cool associated and tyrosine phosphorylated protein 1 Cool-associated and tyrosine-phosphorylated protein 1 G protein coupled receptor kinase interacting ArfGAP 1 G protein coupled receptor kinase interactor 1 G protein-coupled receptor kinase-interactor 1 GIT1 GIT1_HUMAN GRK interacting protein 1 GRK-interacting protein 1

Images

Fig1: Western blot analysis of GIT1 on A549 cell lysates with Rabbit anti-GIT1 antibody (ET7106-91) at 1/500 dilution.

Lysates/proteins at 10 µg/Lane.

Predicted band size: 84 kDa
Observed band size: 84 kDa

Exposure time: 2 minutes;

8% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7106-91) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.

Fig2: Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue with Rabbit anti-GIT1 antibody (ET7106-91) at 1/400 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (ET7106-91) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

	Fig3: Immunohistochemical analysis of paraffin-embedded human pancreas tissue with Rabbit anti-GIT1 antibody (ET7106-91) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (ET7106-91) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig4: Immunohistochemical analysis of paraffin-embedded mouse fallopian tubes tissue using anti-GIT1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (ET7106-91, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig5: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-GIT1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (ET7106-91, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig6: Flow cytometric analysis of GIT1 was done on A549 cells. The cells were fixed, permeabilized and stained with the primary antibody (ET7106-91, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

Fig7: Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue with Rabbit anti-GIT1 antibody (ET7106-91) at 1/400 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (ET7106-91) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.