DKC1 Recombinant Rabbit Monoclonal Antibody [JU34-32]
cat.: ET7106-93
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IF-Cell, IF-Tissue, IHC-P
Clonality: Monoclonal
Clone number: JU34-32
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 58 kDa
Isotype: IgG
Immunogen: Synthetic peptide within Human DKC1 aa 353-402 / 514.
Positive control: A431 cell lysates, A431, human kidney tissue, mouse testis tissue, rat brain tissue, mouse hippocampus tissue, mouse brain tissue.
Subcellular location: Cytoplasm. Nucleus.
Recommended Dilutions:
  WB
  IF-Cell
  IHC-P
1:500-1:1,000
1:50-1:200
1:100-1:400
Uniprot #: SwissProt: O60832 Human | Q9ESX5 Mouse | P40615 Rat
Alternative names: CBF5 CBF5 homolog Cbf5p homolog DKC 1 DKC Dkc1 DKC1_HUMAN DKCX Dyskeratosis congenita 1 Dyskeratosis congenita 1 dyskerin Dyskerin H/ACA ribonucleoprotein complex subunit 4 NAP 57 NAP57 NOLA 4 NOLA4 Nopp140 associated protein of 57 kDa Nopp140-associated protein of 57 kDa Nucleolar protein family A member 4 Nucleolar protein NAP57 snoRNP protein DKC1 XAP 101 XAP101
Images
ET7106-93_1.jpg Fig1: Western blot analysis of DKC1 on A431 cell lysates with Rabbit anti-DKC1 antibody (ET7106-93) at 1/500 dilution.

Lysates/proteins at 10 µg/Lane.

Predicted band size: 58 kDa
Observed band size: 58 kDa

Exposure time: 1 minute;

10% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7106-93) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
ET7106-93_2.jpg Fig2: ICC staining of DKC1 in A431 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (ET7106-93, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ET7106-93_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-DKC1 antibody (ET7106-93) at 1/400 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7106-93) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET7106-93_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded mouse testis tissue with Rabbit anti-DKC1 antibody (ET7106-93) at 1/100 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7106-93) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET7106-93_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-DKC1 antibody (ET7106-93) at 1/400 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7106-93) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET7106-93_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded mouse hippocampus tissue with Rabbit anti-DKC1 antibody (ET7106-93) at 1/400 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7106-93) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET7106-93_7.jpg Fig7: Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-DKC1 antibody (ET7106-93) at 1/400 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7106-93) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.