ILF2 Recombinant Rabbit Monoclonal Antibody [JU50-33]
cat.: ET7106-96
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IP, IHC-P, FC
Clonality: Monoclonal
Clone number: JU50-33
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 43 kDa
Isotype: IgG
Immunogen: Synthetic peptide within Human ILF2 aa 245-294 / 390.
Positive control: SH-SY5Y cell lysates, HL-60 cell lysates, rat brain tissue, human tonsil tissue, human liver cancer tissue, human kidney tissue, mouse testis tissue, SH-SY5Y.
Subcellular location: Nucleus. Cytoplasm.
Recommended Dilutions:
  WB
  IHC-P
  FC
  IP
1:500
1:50-1:200
1:50-1:100
1:10-1:20
Uniprot #: SwissProt: Q12905 Human | Q9CXY6 Mouse | Q7TP98 Rat
Alternative names: HGNC:6037 ILF 2 ilf2 ILF2_HUMAN Interleukin enhancer binding factor 2 Interleukin enhancer binding factor 2, 45kDa Interleukin enhancer-binding factor 2 MGC8391 NF 45 NF45 Nuclear factor of activated T cells 45 kDa Nuclear factor of activated T-cells 45 kDa PRO3063
Images
ET7106-96_1.jpg Fig1: Western blot analysis of ILF2 on different lysates with Rabbit anti-ILF2 antibody (ET7106-96) at 1/500 dilution.

Lane 1: SH-SY5Y cell lysate
Lane 2: HL-60 cell lysate

Lysates/proteins at 10 µg/Lane.

Predicted band size: 43 kDa
Observed band size: 43 kDa

Exposure time: 1 minute;

10% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7106-96) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
ET7106-96_2.jpg Fig2: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-ILF2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7106-96, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET7106-96_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-ILF2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7106-96, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET7106-96_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded human liver carcinoma tissue using anti-ILF2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7106-96, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET7106-96_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-ILF2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7106-96, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET7106-96_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-ILF2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7106-96, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET7106-96_7.jpg Fig7: Flow cytometric analysis of ILF2 was done on SH-SY5Y cells. The cells were fixed, permeabilized and stained with the primary antibody (ET7106-96, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.