PSME1 Recombinant Rabbit Monoclonal Antibody [JU60-33]
cat.: ET7106-98
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IF-Cell, IF-Tissue, IHC-P, IP
Clonality: Monoclonal
Clone number: JU60-33
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 29 kDa
Isotype: IgG
Immunogen: Recombinant protein within Human PSME1 aa 1-155 / 249.
Positive control: MCF-7 cell lysate, rat lung tissue lysate, HepG2, MCF-7, PC-3M, rat kidney tissue, human tonsil tissue, human liver carcinoma tissue, human prostate tissue, mouse small intestine tissue.
Subcellular location: Cytosol. Nucleus.
Recommended Dilutions:
  WB
  IF-Cell
  IHC-P
  IP
1:500-1:1,000
1:50-1:100
1:50-1:200
1:10-1:50
Uniprot #: SwissProt: Q06323 Human | P97371 Mouse | Q63797 Rat
Alternative names: 11S regulator complex alpha subunit 11S regulator complex subunit alpha 29 kD MCP activator subunit 29kD MCP activator subunit Activator of multicatalytic protease subunit 1 IFI5111 IGUP I-5111 Interferon gamma IEF SSP 5111 Interferon gamma inducible protein 5111 Interferon gamma up regulated I 5111 protein Interferon gamma up-regulated I-5111 protein MGC8628 PA28a PA28alpha Proteasome (prosome, macropain) activator subunit 1 (PA28 alpha) Proteasome activator 28 subunit alpha Proteasome activator complex subunit 1 Proteasome activator subunit 1 PSME1 PSME1_HUMAN REG-alpha REGalpha
Images
ET7106-98_1.jpg Fig1: Western blot analysis of PSME1 on different lysates with Rabbit anti-PSME1 antibody (ET7106-98) at 1/500 dilution.

Lane 1: MCF-7 cell lysate
Lane 2: Rat lung tissue lysate (20 µg/Lane)

Lysates/proteins at 10 µg/Lane.

Predicted band size: 29 kDa
Observed band size: 26 kDa

Exposure time: 2 minutes;

15% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7106-98) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
ET7106-98_2.jpg Fig2: ICC staining of PSME1 in HepG2 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (ET7106-98, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ET7106-98_3.jpg Fig3: ICC staining of PSME1 in MCF-7 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (ET7106-98, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ET7106-98_4.jpg Fig4: ICC staining of PSME1 in PC-3M cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (ET7106-98, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ET7106-98_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded rat kidney tissue using anti-PSME1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7106-98, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET7106-98_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-PSME1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7106-98, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET7106-98_7.jpg Fig7: Immunohistochemical analysis of paraffin-embedded human liver carcinoma tissue using anti-PSME1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7106-98, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET7106-98_8.jpg Fig8: Immunohistochemical analysis of paraffin-embedded human prostate tissue using anti-PSME1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7106-98, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET7106-98_9.jpg Fig9: Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue using anti-PSME1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7106-98, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
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