EB3 Recombinant Rabbit Monoclonal Antibody [JU38-30]
cat.: ET7106-99
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, IF-Tissue, IHC-P, FC, IP |
| Clonality: | Monoclonal |
| Clone number: | JU38-30 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 32 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human EB3 aa 165-281 / 281. |
| Positive control: | SH-SY5Y cell lysate, U-87 MG cell lysate, MG-63 cell lysate, mouse brain tissue lysate, rat brain tissue lysate, PC-3M, SH-SY-5Y, A431, human lung cancer tissue, mouse brain tissue, mouse fallopian tubes tissue, rat cerebellum tissue. |
| Subcellular location: | Cytoplasm, cytoskeleton. |
| Recommended Dilutions:
WB IF-Cell IHC-P FC IP |
1:2,000 1:50-1:200 1:50-1:200 1:50-1:100 1-2μg/sample |
| Uniprot #: | SwissProt: Q9UPY8 Human | Q6PER3 Mouse | Q5XIT1 Rat |
| Alternative names: | APC binding protein EB 3 EB1 protein family member 3 EB3 EBF 3 EBF3 EBF3 S End binding protein 3 End-binding protein 3 MAPRE 3 MAPRE3 MARE3_HUMAN Member 3 Microtubule Associated Protein Microtubule associated protein RP/EB family member 3 Microtubule-associated protein RP/EB family member 3 OTTHUMP00000200910 RP 3 RP/EB Family RP3 |
Images
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Fig1:
Western blot analysis of EB3 on different lysates with Rabbit anti-EB3 antibody (ET7106-99) at 1/2,000 dilution. Lane 1: SH-SY5Y cell lysate Lane 2: U-87 MG cell lysate Lane 3: MG-63 cell lysate Lane 4: Mouse brain tissue lysate Lane 5: Rat brain tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 32 kDa Observed band size: 35 kDa Exposure time: Lane 1-3: 30 seconds; Lane 4-5: 8 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7106-99) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2: ICC staining EB3 in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig3: ICC staining EB3 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig4: ICC staining EB3 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig5: Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-EB3 antibody. Counter stained with hematoxylin. |
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Fig6: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-EB3 antibody. Counter stained with hematoxylin. |
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Fig7: Immunohistochemical analysis of paraffin-embedded mouse fallopian tubes tissue using anti-EB3 antibody. Counter stained with hematoxylin. |
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Fig8: Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue using anti-EB3 antibody. Counter stained with hematoxylin. |
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Fig9: Flow cytometric analysis of SH-SY-5Y cells with EB3 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody. |
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Fig10:
EB3 was immunoprecipitated from 0.2 mg U-87 MG cell lysate with ET7106-99 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using ET7106-99 at 1/2,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: U-87 MG cell lysate (input) Lane 2: ET7106-99 IP in U-87 MG cell lysate Lane 3: Rabbit IgG instead of ET7106-99 in U-87 MG cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 1 minute 7 seconds; ECL: K1801 |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.