EB3 Recombinant Rabbit Monoclonal Antibody [JU38-30]
cat.: ET7106-99
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IF-Cell, IF-Tissue, IHC-P, FC
Clonality: Monoclonal
Clone number: JU38-30
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: 32 kDa
Isotype: IgG
Immunogen: Recombinant protein within Human EB3 aa 165-281 / 281.
Positive control: A431, PC-3M, SH-SY-5Y, rat cerebellum tissue, human lung cancer tissue, mouse brain tissue, mouse fallopian tubes tissue, K562.
Subcellular location: Cytoskeleton.
Recommended Dilutions:
  WB
  IF-Cell
  IHC-P
  FC

1:500-1:1,000
1:50-1:200
1:50-1:200
1:50-1:100
Uniprot #: SwissProt: Q9UPY8 Human | Q6PER3 Mouse | Q5XIT1 Rat
Alternative names: APC binding protein EB 3 EB1 protein family member 3 EB3 EBF 3 EBF3 EBF3 S End binding protein 3 End-binding protein 3 MAPRE 3 MAPRE3 MARE3_HUMAN Member 3 Microtubule Associated Protein Microtubule associated protein RP/EB family member 3 Microtubule-associated protein RP/EB family member 3 OTTHUMP00000200910 RP 3 RP/EB Family RP3
Images
ET7106-99_1.jpg Fig1: Western blot analysis of EB3 on different lysates using anti-EB3 antibody at 1/500 dilution.
Positive control:
Lane 1: Mouse brain tissue
Lane 2: K562
ET7106-99_2.jpg Fig2: ICC staining EB3 in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ET7106-99_3.jpg Fig3: ICC staining EB3 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ET7106-99_4.jpg Fig4: ICC staining EB3 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ET7106-99_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-EB3 antibody. Counter stained with hematoxylin.
ET7106-99_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-EB3 antibody. Counter stained with hematoxylin.
ET7106-99_7.jpg Fig7: Immunohistochemical analysis of paraffin-embedded mouse fallopian tubes tissue using anti-EB3 antibody. Counter stained with hematoxylin.
ET7106-99_8.jpg Fig8: Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue using anti-EB3 antibody. Counter stained with hematoxylin.
ET7106-99_9.jpg Fig9: Flow cytometric analysis of SH-SY-5Y cells with EB3 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.