EB3 Recombinant Rabbit Monoclonal Antibody [JU38-30]
cat.: ET7106-99
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, IF-Tissue, IHC-P, FC |
| Clonality: | Monoclonal |
| Clone number: | JU38-30 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | 32 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human EB3 aa 165-281 / 281. |
| Positive control: | A431, PC-3M, SH-SY-5Y, rat cerebellum tissue, human lung cancer tissue, mouse brain tissue, mouse fallopian tubes tissue, K562. |
| Subcellular location: | Cytoskeleton. |
| Recommended Dilutions:
WB IF-Cell IHC-P FC |
1:500-1:1,000 1:50-1:200 1:50-1:200 1:50-1:100 |
| Uniprot #: | SwissProt: Q9UPY8 Human | Q6PER3 Mouse | Q5XIT1 Rat |
| Alternative names: | APC binding protein EB 3 EB1 protein family member 3 EB3 EBF 3 EBF3 EBF3 S End binding protein 3 End-binding protein 3 MAPRE 3 MAPRE3 MARE3_HUMAN Member 3 Microtubule Associated Protein Microtubule associated protein RP/EB family member 3 Microtubule-associated protein RP/EB family member 3 OTTHUMP00000200910 RP 3 RP/EB Family RP3 |
Images
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Fig1:
Western blot analysis of EB3 on different lysates using anti-EB3 antibody at 1/500 dilution. Positive control: Lane 1: Mouse brain tissue Lane 2: K562 |
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Fig2: ICC staining EB3 in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig3: ICC staining EB3 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig4: ICC staining EB3 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig5: Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-EB3 antibody. Counter stained with hematoxylin. |
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Fig6: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-EB3 antibody. Counter stained with hematoxylin. |
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Fig7: Immunohistochemical analysis of paraffin-embedded mouse fallopian tubes tissue using anti-EB3 antibody. Counter stained with hematoxylin. |
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Fig8: Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue using anti-EB3 antibody. Counter stained with hematoxylin. |
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Fig9: Flow cytometric analysis of SH-SY-5Y cells with EB3 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.