VPS35 Recombinant Rabbit Monoclonal Antibody [JB33-82]
cat.: ET7107-01
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB, IHC-P, FC |
| Clonality: | Monoclonal |
| Clone number: | JB33-82 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 92 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human VPS35 aa 80-220 / 796. |
| Positive control: | U-937 cell lysate, A549 cell lysate, NIH/3T3 cell lysate, C2C12 cell lysate, mouse colon tissue lysate, human colon cancer tissue, human kidney tissue, human placenta tissue, mouse colon tissue, 293T. |
| Subcellular location: | Cytoplasm. Membrane. Endosome. |
| Recommended Dilutions:
WB IHC-P FC |
1:1,000 1:100-1:300 1:50-1:100 |
| Uniprot #: | SwissProt: Q96QK1 Human | Q9EQH3 Mouse |
| Alternative names: | DKFZp434E1211 DKFZp434P1672 FLJ10752 FLJ13588 FLJ20388 hVPS35 Maternal embryonic 3 Maternal-embryonic 3 MEM 3 MEM3 PARK17 TCCCTA00141 Vacuolar protein sorting 35 (yeast) Vacuolar protein sorting 35 Vacuolar protein sorting 35 homolog Vacuolar protein sorting associated protein 35 Vacuolar protein sorting-associated protein 35 Vesicle protein sorting 35 VPS 35 VPS35 VPS35_HUMAN |
Images
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Fig1:
Western blot analysis of VPS35 on different lysates with Rabbit anti-VPS35 antibody (ET7107-01) at 1/1,000 dilution. Lane 1: U-937 cell lysate (20 µg/Lane) Lane 2: A549 cell lysate (20 µg/Lane) Lane 3: NIH/3T3 cell lysate (20 µg/Lane) Lane 4: C2C12 cell lysate (20 µg/Lane) Lane 5: Mouse colon tissue lysate (40 µg/Lane) Predicted band size: 92 kDa Observed band size: 80 kDa Exposure time: 30 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7107-01) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-VPS35 antibody. Counter stained with hematoxylin. |
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Fig3: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-VPS35 antibody. Counter stained with hematoxylin. |
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Fig4: Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-VPS35 antibody. Counter stained with hematoxylin. |
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Fig5: Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-VPS35 antibody. Counter stained with hematoxylin. |
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Fig6: Flow cytometric analysis of 293T cells with VPS35 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.