RING2 Recombinant Rabbit Monoclonal Antibody [JB38-41]
cat.: ET7107-07
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat, Monkey |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JB38-41 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 38 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human RING2 aa 83-256 . |
| Positive control: | HeLa cell lysate, COS-1 cell lysate, NIH/3T3 cell lysate, RAW264.7 cell lysate, C6 cell lysate, PC-12 cell lysate, human breast carcinoma tissue, mouse liver tissue, rat liver tissue. |
| Subcellular location: | Chromosome. Nucleus. |
| Recommended Dilutions:
WB IHC-P |
1:5,000 1:200 |
| Uniprot #: | SwissProt: Q99496 Human | Q9CQJ4 Mouse | Q4KLY4 Rat |
| Alternative names: | BAP 1 BAP1 DING DinG protein E3 ubiquitin protein ligase RING 2 E3 ubiquitin protein ligase RING2 E3 ubiquitin-protein ligase RING2 HIP2 interacting protein 3 HIP2-interacting protein 3 HIPI 3 HIPI3 Huntingtin interacting protein 2 interacting protein 3 Huntingtin-interacting protein 2-interacting protein 3 OTTHUMP00000060668 Polycomb M33 interacting protein Ring 1B Polycomb M33 interacting protein Ring1B Protein DinG RING 1B RING 2 RING finger protein 1B RING finger protein 2 RING finger protein BAP 1 RING finger protein BAP-1 RING finger protein BAP1 RING1b RING2_HUMAN RNF 2 Rnf2 |
Images
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Fig1:
Western blot analysis of RING2 on different lysates with Rabbit anti-RING2 antibody (ET7107-07) at 1/5,000 dilution. Lane 1: HeLa cell lysate Lane 2: COS-1 cell lysate Lane 3: NIH/3T3 cell lysate Lane 4: RAW264.7 cell lysate Lane 5: C6 cell lysate Lane 6: PC-12 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 38 kDa Observed band size: 40 kDa Exposure time: 13 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7107-07) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue with Rabbit anti-RING2 antibody (ET7107-07) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7107-07) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded mouse liver tissue with Rabbit anti-RING2 antibody (ET7107-07) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7107-07) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Immunohistochemical analysis of paraffin-embedded rat liver tissue with Rabbit anti-RING2 antibody (ET7107-07) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7107-07) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.