ET7107-07

Product Type:	Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity:	Human, Mouse, Rat
Applications:	WB, IHC-P
Clonality:	Monoclonal
Clone number:	JB38-41

Form:

Liquid

Storage condition:

Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.

Storage buffer:

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration:

1ug/ul

Purification:

Protein A affinity purified.

Molecular weight:

Predicted band size: 38 kDa

Isotype:

IgG

Immunogen:

Recombinant protein within Human RING2 aa 83-256 .

Positive control:

K562 cell lysate, human placenta tissue, human breast carcinoma tissue.

Subcellular location:

Chromosome. Nucleus.

Recommended Dilutions:
WB
IHC-P

1:500-1:1,000
1:50-1:1,000

Uniprot #:

SwissProt: Q99496 Human

Alternative names:

BAP 1 BAP1 DING DinG protein E3 ubiquitin protein ligase RING 2 E3 ubiquitin protein ligase RING2 E3 ubiquitin-protein ligase RING2 HIP2 interacting protein 3 HIP2-interacting protein 3 HIPI 3 HIPI3 Huntingtin interacting protein 2 interacting protein 3 Huntingtin-interacting protein 2-interacting protein 3 OTTHUMP00000060668 Polycomb M33 interacting protein Ring 1B Polycomb M33 interacting protein Ring1B Protein DinG RING 1B RING 2 RING finger protein 1B RING finger protein 2 RING finger protein BAP 1 RING finger protein BAP-1 RING finger protein BAP1 RING1b RING2_HUMAN RNF 2 Rnf2

	Fig1: Western blot analysis of RING2 on k562 cell using anti-RING2 antibody at 1/500 dilution.
	Fig2: Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-RING2 antibody. Counter stained with hematoxylin.
	Fig3: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue with Rabbit anti-RING2 antibody (ET7107-07) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (ET7107-07) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.