RING2 Recombinant Rabbit Monoclonal Antibody [JB38-41]
cat.: ET7107-07
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IHC-P
Clonality: Monoclonal
Clone number: JB38-41
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 38 kDa
Isotype: IgG
Immunogen: Recombinant protein within Human RING2 aa 83-256 .
Positive control: K562 cell lysate, human placenta tissue, human breast carcinoma tissue.
Subcellular location: Chromosome. Nucleus.
Recommended Dilutions:
  WB
  IHC-P

1:500-1:1,000
1:50-1:1,000
Uniprot #: SwissProt: Q99496 Human
Alternative names: BAP 1 BAP1 DING DinG protein E3 ubiquitin protein ligase RING 2 E3 ubiquitin protein ligase RING2 E3 ubiquitin-protein ligase RING2 HIP2 interacting protein 3 HIP2-interacting protein 3 HIPI 3 HIPI3 Huntingtin interacting protein 2 interacting protein 3 Huntingtin-interacting protein 2-interacting protein 3 OTTHUMP00000060668 Polycomb M33 interacting protein Ring 1B Polycomb M33 interacting protein Ring1B Protein DinG RING 1B RING 2 RING finger protein 1B RING finger protein 2 RING finger protein BAP 1 RING finger protein BAP-1 RING finger protein BAP1 RING1b RING2_HUMAN RNF 2 Rnf2
Images
ET7107-07_1.jpg Fig1: Western blot analysis of RING2 on k562 cell using anti-RING2 antibody at 1/500 dilution.
ET7107-07_2.jpg Fig2: Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-RING2 antibody. Counter stained with hematoxylin.
ET7107-07_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue with Rabbit anti-RING2 antibody (ET7107-07) at 1/200 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7107-07) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.