GABA A Receptor alpha 5 Recombinant Rabbit Monoclonal Antibody [JB34-19]
cat.: ET7107-08
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, IF-Tissue, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JB34-19 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | 52 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human GABA A Receptor alpha 5 aa 1-240 / 462. |
| Positive control: | A549 cell lysate, 293T cell lysate, rat brain tissue lysate, SH-SY-5Y cell, 293T cell, A549 cell, rat brain tissue, mouse brain tissue. |
| Subcellular location: | Plasma membrane. |
| Recommended Dilutions:
WB IF-Cell IF-Tissue IHC-P |
1:500-1:2,000 1:50-1:200 1:50-1:200 1:50-1:200 |
| Uniprot #: | SwissProt: P31644 Human | Q8BHJ7 Mouse | P19969 Rat |
| Alternative names: | GAA 5 GAA5 GABA(A) receptor subunit alpha-5 GABRA 5 Gabra5 Gamma aminobutyric acid GABA A receptor alpha 5 Gamma aminobutyric acid GABA A receptor alpha 5 precursor Gamma aminobutyric acid receptor alpha 5 subunit precursor GABA A receptor Gamma-aminobutyric acid receptor subunit alpha-5 GBRA5_HUMAN GC138184 |
Images
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Fig1:
Western blot analysis of GABA A Receptor alpha 5 on different lysates using anti-GABA A Receptor alpha 5 antibody at 1/1,000 dilution. Positive control: Lane 1: A549 cell lysate Lane 2: 293T cell lysate Lane 3: Rat brain tissue lysate |
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Fig2: ICC staining GABA A Receptor alpha 5 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig3: ICC staining GABA A Receptor alpha 5 in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig4: ICC staining GABA A Receptor alpha 5 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig5: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-GABA A Receptor alpha 5 antibody. Counter stained with hematoxylin. |
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Fig6: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-GABA A Receptor alpha 5 antibody. Counter stained with hematoxylin. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.