GABRA5 Recombinant Rabbit Monoclonal Antibody [JB34-19]
cat.: ET7107-08
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Tissue, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JB34-19 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 52 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human GABRA5 aa 1-240 / 462. |
| Positive control: | A549 cell lysate, Neuro-2a cell lysate, C6 cell lysate, Mouse brain tissue lysate, mouse brain tissue, rat brain tissue, mouse cerebellum tissue. |
| Subcellular location: | Postsynaptic cell membrane, Cell membrane. |
| Recommended Dilutions:
WB IF-Tissue IHC-P |
1:2,000 1:500 1:1,000 |
| Uniprot #: | SwissProt: P31644 Human | Q8BHJ7 Mouse | P19969 Rat |
| Alternative names: | GAA 5 GAA5 GABA(A) receptor subunit alpha-5 GABRA 5 Gabra5 Gamma aminobutyric acid GABA A receptor alpha 5 Gamma aminobutyric acid GABA A receptor alpha 5 precursor Gamma aminobutyric acid receptor alpha 5 subunit precursor GABA A receptor Gamma-aminobutyric acid receptor subunit alpha-5 GBRA5_HUMAN GC138184 |
Images
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Fig1:
Western blot analysis of GABRA5 on different lysates with Rabbit anti-GABRA5 antibody (ET7107-08) at 1/2,000 dilution. Lane 1: A549 cell lysate (20 µg/Lane) Lane 2: Neuro-2a cell lysate (20 µg/Lane) Lane 3: C6 cell lysate (20 µg/Lane) Lane 4: Mouse brain tissue lysate (40 µg/Lane) Predicted band size: 52 kDa Observed band size: 70 kDa Exposure time: 1 minute; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7107-08) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-GABRA5 antibody (ET7107-08) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7107-08) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-GABRA5 antibody (ET7107-08) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7107-08) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Immunofluorescence analysis of paraffin-embedded mouse cerebellum tissue labeling GABRA5 with Rabbit anti-GABRA5 antibody (ET7107-08) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (ET7107-08, green) at 1/500 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.