Versican Recombinant Rabbit Monoclonal Antibody [JB42-32]
cat.: ET7107-09
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | IHC-P, WB, IF-Tissue |
| Clonality: | Monoclonal |
| Clone number: | JB42-32 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | 373/265/182/74 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human Versican aa 3,160-3,396 / 3,396. |
| Positive control: | Hela cell lysates, human spleen tissue, mouse brain tissue. |
| Subcellular location: | Extracellular matrix. Secreted. |
| Recommended Dilutions:
IHC-P WB IF-Tissue |
1:50-1:1,000 1:1,000 1;200 |
| Uniprot #: | SwissProt: P13611 Human | Q62059 Mouse |
| Alternative names: | Chondroitin sulfate proteoglycan 2 Chondroitin sulfate proteoglycan core protein 2 Chondroitin sulfate proteoglycan core protein, cartilage CSPG2 CSPG2_HUMAN ERVR GHAP Glial hyaluronate binding protein Glial hyaluronate-binding protein Large fibroblast proteoglycan PG-M PGM VCAN Versican Versican core protein Versican proteoglycan WGN 1 WGN WGN1 |
Images
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Fig1:
Western blot analysis of Versican on different lysates with Rabbit anti-Versican antibody (ET7107-09) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: Mouse brain tissue lysate Lane 3: Mouse hippocampus tissue lysate Lane 4: Rat brain tissue lysate Lysates/proteins at 20(cell lysate)/40(tissue lysate）µg/Lane. Predicted band size: 370 kDa Observed band size: 370 kDa Exposure time: 3 minutes 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7107-09) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2: Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-Versican antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7107-09, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-Versican antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7107-09, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-Versican antibody (ET7107-09) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7107-09) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.