LRG1 Recombinant Rabbit Monoclonal Antibody [JB34-31]
cat.: ET7107-11
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IHC-P, IP |
| Clonality: | Monoclonal |
| Clone number: | JB34-31 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 38 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human LRG1 aa 261-310 / 347. |
| Positive control: | Human serum lysates, human kidney tissue. |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
WB IHC-P IP |
1:500-1:2,000 1:50-1:200 1:10-1:50 |
| Uniprot #: | SwissProt: P02750 Human |
| Alternative names: | 1300008B03Rik 2310031E04Rik A2GL A2GL_HUMAN HMFT1766 Leucine rich alpha 2 glycoprotein Leucine-rich alpha-2-glycoprotein 1 Leucine-rich alpha-2-glycoprotein Leucine-rich alpha-2-glycoprotein precursor LRG LRG1 |
Images
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Fig1:
Western blot analysis of LRG1 on human serum lysates with Rabbit anti-LRG1 antibody (ET7107-11) at 1/500 dilution. Lysates/proteins at 10 µg/Lane. Predicted band size: 38 kDa Observed band size: 43 kDa Exposure time: 30 seconds; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7107-11) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
|
Fig2: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-LRG1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7107-11, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.