SMARCC1 Recombinant Rabbit Monoclonal Antibody [JB43-43]
cat.: ET7107-12
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse
Applications: WB, IF-Cell, IF-Tissue, IHC-P, FC, IP
Clonality: Monoclonal
Clone number: JB43-43
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 123 kDa
Isotype: IgG
Immunogen: Recombinant protein within Human SMARCC1 aa 680-820 / 1,105.
Positive control: HL-60 cell lysate, mouse thymus tissue lysate, NIH/3T3 cell lysate, 293T, mouse testis tissue, K562, human lymph node tissue, human esophagus tissue, human small intestine tissue.
Subcellular location: Nucleus. Cytoplasm.
Recommended Dilutions:
  WB
  IF-Cell
  IHC-P
  FC
  IP
1:500
1:50
1:50-1:400
1:500-1:1,000
1:10-1:50
Uniprot #: SwissProt: Q92922 Human | P97496 Mouse
Alternative names: AI115498 BAF 155 BAF155 BRG 1 associated factor 155 BRG1 associated factor 155 BRG1-associated factor 155 Chromatin remodeling complex BAF155 subunit CRACC 1 CRACC1 Mammalian chromatin remodeling complex BRG 1 associated factor 155 Mammalian chromatin remodeling complex BRG1 associated factor 155 Rsc 8 Rsc8 SMARC C1 SMARCC 1 SMARCC1 SMRC1_HUMAN SRG 3 SRG3 SWI 3 SWI/SNF complex 155 kDa subunit SWI/SNF complex subunit SMARCC1 SWI/SNF related matrix associated actin dependent regulator of chromatin c1 SWI/SNF related matrix associated actin dependent regulator of chromatin subfamily c member 1 SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily C member 1 SWI3
Images
ET7107-12_1.jpg Fig1: Western blot analysis of SMARCC1 on different lysates with Rabbit anti-SMARCC1 antibody (ET7107-12) at 1/500 dilution.

Lane 1: HL-60 cell lysate (10 µg/Lane)
Lane 2: Mouse thymus tissue lysate (20 µg/Lane)

Predicted band size: 123 kDa
Observed band size: 155 kDa

Exposure time: 1 minute;

6% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7107-12) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.
ET7107-12_2.jpg Fig2: Western blot analysis of SMARCC1 on different lysates with Rabbit anti-SMARCC1 antibody (ET7107-12) at 1/500 dilution.

Lane 1: NIH/3T3 cell lysate (20 µg/Lane)
Lane 2: Mouse thymus tissue lysate (40 µg/Lane)

Predicted band size: 123 kDa
Observed band size: 150 kDa

Exposure time: 1 minute 2 seconds
4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7107-12) at 1/500 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
ET7107-12_3.jpg Fig3: ICC staining of SMARCC1 in 293T cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (ET7107-12, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ET7107-12_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-SMARCC1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7107-12, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET7107-12_5.jpg Fig5: Flow cytometric analysis of SMARCC1 was done on K562 cells. The cells were fixed, permeabilized and stained with the primary antibody (ET7107-12, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
ET7107-12_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded human lymph node tissue using anti-SMARCC1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7107-12, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET7107-12_7.jpg Fig7: Immunohistochemical analysis of paraffin-embedded human esophagus tissue using anti-SMARCC1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7107-12, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET7107-12_8.jpg Fig8: Immunohistochemical analysis of paraffin-embedded human small intestine tissue using anti-SMARCC1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7107-12, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.