MCM5 Recombinant Rabbit Monoclonal Antibody [JB31-34]
cat.: ET7107-15
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JB31-34 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 82 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human MCM5 aa 620-734 / 734. |
| Positive control: | SiHa cell lysate, HL-60 cell lysate, human tonsil tissue, human placenta tissue, mouse testis tissue, mouse testis tissue lysates, human lymph nodes tissue. |
| Subcellular location: | Nucleus. Cytosol. |
| Recommended Dilutions:
WB IHC-P |
1:500-1:1,000 1:50-1:400 |
| Uniprot #: | SwissProt: P33992 Human | P49718 Mouse |
| Alternative names: | CDC 46 CDC46 CDC46 homolog Cell division cycle 46 DNA replication licensing factor DNA replication licensing factor MCM 5 DNA replication licensing factor MCM5 MCM 5 MCM5 MCM5_HUMAN MGC5315 Minichromosome maintenance complex component 5 Minichromosome maintenance deficient (S. cerevisiae) 5 Minichromosome maintenance deficient 5 Minichromosome maintenance deficient protein 5 P1 CDC46 P1-CDC46 |
Images
|
Fig1:
Western blot analysis of MCM5 on different lysates with Rabbit anti-MCM5 antibody (ET7107-15) at 1/500 dilution. Lane 1: SiHa cell lysate Lane 2: HL-60 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 82 kDa Observed band size: 82 kDa Exposure time: 30 seconds; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7107-15) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
|
Fig2: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-MCM5 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7107-15, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig3: Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-MCM5 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7107-15, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig4: Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-MCM5 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7107-15, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig5:
Western blot analysis of MCM5 on mouse testis tissue lysates with Rabbit anti-MCM5 antibody (ET7107-15) at 1/500 dilution. Lysates/proteins at 20 µg/Lane. Predicted band size: 82 kDa Observed band size: 82 kDa Exposure time: 1 minute; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7107-15) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
|
Fig6:
Immunohistochemical analysis of paraffin-embedded human lymph nodes tissue with Rabbit anti-MCM5 antibody (ET7107-15) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7107-15) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.