MCM5 Recombinant Rabbit Monoclonal Antibody [JB31-34]
cat.: ET7107-15
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse
Applications: WB, IHC-P
Clonality: Monoclonal
Clone number: JB31-34
Form: Liquid
Storage condition: Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 82 kDa
Isotype: IgG
Immunogen: Recombinant protein within Human MCM5 aa 620-734 / 734.
Positive control: A431 cell lysate, HEK-293 cell lysate, SiHa cell lysate, mouse testis tissue lysate, human tonsil tissue, human placenta tissue, mouse testis tissue, human lymph nodes tissue.
Subcellular location: Nucleus. Cytosol.
Recommended Dilutions:
  WB
  IHC-P
1:500-1:1,000
1:50-1:400
Uniprot #: SwissProt: P33992 Human | P49718 Mouse
Alternative names: CDC 46 CDC46 CDC46 homolog Cell division cycle 46 DNA replication licensing factor DNA replication licensing factor MCM 5 DNA replication licensing factor MCM5 MCM 5 MCM5 MCM5_HUMAN MGC5315 Minichromosome maintenance complex component 5 Minichromosome maintenance deficient (S. cerevisiae) 5 Minichromosome maintenance deficient 5 Minichromosome maintenance deficient protein 5 P1 CDC46 P1-CDC46
Images
ET7107-15_1.jpg Fig1: Western blot analysis of MCM5 on different lysates with Rabbit anti-MCM5 antibody (ET7107-15) at 1/5,000 dilution.

Lane 1: A431 (Human epidermoid carcinoma skin squamous cell) cell lysate
Lane 2: HEK-293 (Human embryonic kidney cell) cell lysate
Lane 3: SiHa (Human cervical cancersSquamous cell) cell lysate

Lysates/proteins at 15 µg/Lane.
Exposure time: 46 seconds; ECL: K1801

Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: ET7107-15, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature

Predicted band size: 82.3 kDa
Observed band size: 82 kDa
ET7107-15_2.jpg Fig2: Western blot analysis of MCM5 on mouse testis tissue lysates with Rabbit anti-MCM5 antibody (ET7107-15) at 1/500 dilution.

Lysates/proteins at 20 µg/Lane.

Predicted band size: 82 kDa
Observed band size: 82 kDa

Exposure time: 1 minute;

8% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7107-15) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.
ET7107-15_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-MCM5 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7107-15, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET7107-15_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-MCM5 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7107-15, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET7107-15_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-MCM5 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7107-15, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET7107-15_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded human lymph nodes tissue with Rabbit anti-MCM5 antibody (ET7107-15) at 1/400 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7107-15) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.