OGT Recombinant Rabbit Monoclonal Antibody [JB44-39]
cat.: ET7107-17
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IF-Cell, IF-Tissue, IHC-P, FC
Clonality: Monoclonal
Clone number: JB44-39
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 117 kDa
Isotype: IgG
Immunogen: Synthetic peptide within Human OGT aa 997-1,046 / 1,046.
Positive control: SiHa cell lysate, Hela cell lysate, A431, Hela, PC-3M, rat epididymis tissue, human liver tissue, human kidney tissue, mouse testis tissue.
Subcellular location: Nucleus. Cytoplasm. Membrane.
Recommended Dilutions:
  WB
  IF-Cell
  IHC-P
  FC

1:500
1:500-1:1,000
1:50-1:200
1:50-1:100
Uniprot #: SwissProt: O15294 Human | Q8CGY8 Mouse | P56558 Rat
Alternative names: FLJ23071 GlcNAc transferase HRNT1 MGC22921 O GlcNAc O GlcNAc transferase p110 subunit O GlcNAc transferase subunit p110 O linked N acetylglucosamine (GlcNAc) transferase (UDP N acetylglucosamine:polypeptide N acetylglucosaminyl transferase) O linked N acetylglucosamine (GlcNAc) transferase O linked N acetylglucosamine transferase 110 kDa subunit O-GlcNAc transferase subunit p110 O-linked N-acetylglucosamine transferase 110 kDa subunit ogt OGT1_HUMAN UDP N acetylglucosamine peptide N acetylglucosaminyltransferase 110 kDa subunit UDP N acetylglucosamine peptide N acetylglucosaminyltransferase GlcNAc transferase UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase 110 kDa subunit UDP-N-acetylglucosamine:polypeptide-N-acetylglucosaminyl transferase Uridinediphospho N acetylglucosamine:polypeptide beta N acetylglucosaminyl transferase
Images
ET7107-17_1.jpg Fig1: Western blot analysis of OGT on different lysates with Rabbit anti-OGT antibody (ET7107-17) at 1/500 dilution.

Lane 1: SiHa cell lysate
Lane 2: Hela cell lysate

Lysates/proteins at 10 µg/Lane.

Predicted band size: 117 kDa
Observed band size: 117 kDa

Exposure time: 2 minutes;

6% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7107-17) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.
ET7107-17_2.jpg Fig2: ICC staining OGT in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ET7107-17_3.jpg Fig3: ICC staining OGT in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ET7107-17_4.jpg Fig4: ICC staining OGT in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ET7107-17_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded rat epididymis tissue using anti-OGT antibody. Counter stained with hematoxylin.
ET7107-17_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-OGT antibody. Counter stained with hematoxylin.
ET7107-17_7.jpg Fig7: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-OGT antibody. Counter stained with hematoxylin.
ET7107-17_8.jpg Fig8: Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-OGT antibody. Counter stained with hematoxylin.
ET7107-17_9.jpg Fig9: Flow cytometric analysis of Hela cells with OGT antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.