Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
---|---|
Species reactivity: | Human, Mouse, Rat |
Applications: | WB, IF-Cell, IF-Tissue, IHC-P, FC |
Clonality: | Monoclonal |
Clone number: | JB44-39 |
Form: | Liquid |
Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
Concentration: | 1ug/ul |
Purification: | Protein A affinity purified. |
Molecular weight: | Predicted band size: 117 kDa |
Isotype: | IgG |
Immunogen: | Synthetic peptide within Human OGT aa 997-1,046 / 1,046. |
Positive control: | SiHa cell lysate, Hela cell lysate, A431, Hela, PC-3M, rat epididymis tissue, human liver tissue, human kidney tissue, mouse testis tissue. |
Subcellular location: | Nucleus. Cytoplasm. Membrane. |
Recommended Dilutions:
WB IF-Cell IHC-P FC |
1:500 1:500-1:1,000 1:50-1:200 1:50-1:100 |
Uniprot #: | SwissProt: O15294 Human | Q8CGY8 Mouse | P56558 Rat |
Alternative names: | FLJ23071 GlcNAc transferase HRNT1 MGC22921 O GlcNAc O GlcNAc transferase p110 subunit O GlcNAc transferase subunit p110 O linked N acetylglucosamine (GlcNAc) transferase (UDP N acetylglucosamine:polypeptide N acetylglucosaminyl transferase) O linked N acetylglucosamine (GlcNAc) transferase O linked N acetylglucosamine transferase 110 kDa subunit O-GlcNAc transferase subunit p110 O-linked N-acetylglucosamine transferase 110 kDa subunit ogt OGT1_HUMAN UDP N acetylglucosamine peptide N acetylglucosaminyltransferase 110 kDa subunit UDP N acetylglucosamine peptide N acetylglucosaminyltransferase GlcNAc transferase UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase 110 kDa subunit UDP-N-acetylglucosamine:polypeptide-N-acetylglucosaminyl transferase Uridinediphospho N acetylglucosamine:polypeptide beta N acetylglucosaminyl transferase |
Fig1:
Western blot analysis of OGT on different lysates with Rabbit anti-OGT antibody (ET7107-17) at 1/500 dilution. Lane 1: SiHa cell lysate Lane 2: Hela cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 117 kDa Observed band size: 117 kDa Exposure time: 2 minutes; 6% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7107-17) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
|
Fig2: ICC staining OGT in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. | |
Fig3: ICC staining OGT in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
Fig4: ICC staining OGT in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. | |
Fig5: Immunohistochemical analysis of paraffin-embedded rat epididymis tissue using anti-OGT antibody. Counter stained with hematoxylin. | |
Fig6: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-OGT antibody. Counter stained with hematoxylin. | |
Fig7: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-OGT antibody. Counter stained with hematoxylin. |
Fig8: Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-OGT antibody. Counter stained with hematoxylin. | |
Fig9: Flow cytometric analysis of Hela cells with OGT antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody |