Mast Cell Chymase Recombinant Rabbit Monoclonal Antibody [JB74-32]
cat.: ET7107-19
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JB74-32 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 27 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human Mast Cell Chymase aa 198-247 / 247. |
| Positive control: | Mouse spleen tissue lysate, human lymph nodes tissue, human skin tissue, human tonsil tissue. |
| Subcellular location: | Secreted, Cytoplasmic granule. |
| Recommended Dilutions:
WB IHC-P |
1:500 1:200-1:20,000 |
| Uniprot #: | SwissProt: P23946 Human | P21844 Mouse | P50339 Rat |
| Alternative names: | Alpha-chymase Chymase 1 Chymase 1 mast cell chymase 1 preproprotein transcript E chymase 1 preproprotein transcript I Chymase Chymase, heart Chymase, mast cell CMA1 CMA1_HUMAN CYH CYM EC 3.4.21.39 Mast cell chymase 1 Mast cell protease 3 Mast cell protease 5 Mast cell protease I Mast cell protease III Mcp-5 MCP3P Mcpt5 MCT1 MGC119890 MGC119891 MMCP-5 |
Images
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Fig1:
Western blot analysis of Mast Cell Chymase on mouse spleen tissue lysates with Rabbit anti-Mast Cell Chymase antibody (ET7107-19) at 1:1000 dilution. Lysates/proteins at 10 µg/Lane. Exposure time: 3 minutes; ECL: K1802 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: ET7107-19, 1/1,000 in 5% NFDM/TBST, overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 27.3 kDa Observed band size: 27 kDa |
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Fig2:
Immunohistochemical analysis of paraffin-embedded human lymph nodes tissue with Rabbit anti-Mast Cell Chymase antibody (ET7107-19) at 1/20,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7107-19) at 1/20,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3: Immunohistochemical analysis of paraffin-embedded human skin tissue using anti-Mast Cell Chymase antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7107-19, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Mast Cell Chymase antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7107-19, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.