Wnt2b Recombinant Rabbit Monoclonal Antibody [JB48-34]
cat.: ET7107-20
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IHC-P, IF-Cell, IF-Tissue, FC
Clonality: Monoclonal
Clone number: JB48-34
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 44 kDa
Isotype: IgG
Immunogen: Recombinant protein within Human Wnt2b aa 175-355 .
Positive control: Rat testis tissue lysate, Hela, LOVO, SHSY-5Y, rat testis tissue, human kidney tissue, mouse kidney tissue.
Subcellular location: Secreted. Extracellular region.
Recommended Dilutions:
  WB
  IF-Cell
  IHC-P
  FC

1:500-1:1,000
1:100-1:500
1:50-1:200
1:50-1:100
Uniprot #: SwissProt: Q93097 Human | O70283 Mouse | G3V7U3 Rat
Alternative names: Protein Wnt-13 Protein Wnt-2b Wingless type MMTV integration site family, member 13 Wingless type MMTV integration site family, member 2B WNT13 Wnt2b WNT2B_HUMAN XWNT2 XWNT2, Xenopus, homolog of
Images
ET7107-20_1.jpg Fig1: Western blot analysis of Wnt2b on rat testis tissue lysates with Rabbit anti-Wnt2b antibody (ET7107-20) at 1/500 dilution.

Lysates/proteins at 20 µg/Lane.

Predicted band size: 44 kDa
Observed band size: 44 kDa

Exposure time: 2 minutes;

10% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7107-20) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.
ET7107-20_2.jpg Fig2: ICC staining Wnt2b in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ET7107-20_3.jpg Fig3: ICC staining Wnt2b in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ET7107-20_4.jpg Fig4: ICC staining Wnt2b in SHSY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ET7107-20_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded rat testis tissue using anti-Wnt2b antibody. Counter stained with hematoxylin.
ET7107-20_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Wnt2b antibody. Counter stained with hematoxylin.
ET7107-20_7.jpg Fig7: Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-Wnt2b antibody. Counter stained with hematoxylin.
ET7107-20_8.jpg Fig8: Flow cytometric analysis of LOVO cells with Wnt2b antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.