Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
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Species reactivity: | Human, Mouse, Rat |
Applications: | WB, IHC-P, IF-Cell, IF-Tissue, FC |
Clonality: | Monoclonal |
Clone number: | JB48-34 |
Form: | Liquid |
Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
Concentration: | 1ug/ul |
Purification: | Protein A affinity purified. |
Molecular weight: | Predicted band size: 44 kDa |
Isotype: | IgG |
Immunogen: | Recombinant protein within Human Wnt2b aa 175-355 . |
Positive control: | Rat testis tissue lysate, Hela, LOVO, SHSY-5Y, rat testis tissue, human kidney tissue, mouse kidney tissue. |
Subcellular location: | Secreted. Extracellular region. |
Recommended Dilutions:
WB IF-Cell IHC-P FC |
1:500-1:1,000 1:100-1:500 1:50-1:200 1:50-1:100 |
Uniprot #: | SwissProt: Q93097 Human | O70283 Mouse | G3V7U3 Rat |
Alternative names: | Protein Wnt-13 Protein Wnt-2b Wingless type MMTV integration site family, member 13 Wingless type MMTV integration site family, member 2B WNT13 Wnt2b WNT2B_HUMAN XWNT2 XWNT2, Xenopus, homolog of |
Fig1:
Western blot analysis of Wnt2b on rat testis tissue lysates with Rabbit anti-Wnt2b antibody (ET7107-20) at 1/500 dilution. Lysates/proteins at 20 µg/Lane. Predicted band size: 44 kDa Observed band size: 44 kDa Exposure time: 2 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7107-20) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
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Fig2: ICC staining Wnt2b in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. | |
Fig3: ICC staining Wnt2b in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. | |
Fig4: ICC staining Wnt2b in SHSY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
Fig5: Immunohistochemical analysis of paraffin-embedded rat testis tissue using anti-Wnt2b antibody. Counter stained with hematoxylin. | |
Fig6: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Wnt2b antibody. Counter stained with hematoxylin. | |
Fig7: Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-Wnt2b antibody. Counter stained with hematoxylin. | |
Fig8: Flow cytometric analysis of LOVO cells with Wnt2b antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody. |