VPS26 Recombinant Rabbit Monoclonal Antibody [JB93-41]
cat.: ET7107-21
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IHC-P, IP
Clonality: Monoclonal
Clone number: JB93-41
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 38 kDa
Isotype: IgG
Immunogen: Recombinant protein within Human VPS26 aa 210-327 / 327.
Positive control: Human liver tissue lysate, Hela cell lysate, rat kidney tissue, human tonsil tissue, mouse testis tissue.
Subcellular location: Endosome. Cytoplasm.
Recommended Dilutions:
  WB
  IHC-P
  IP

1:500-1:2,000
1:50-1:200
1:10-1:50
Uniprot #: SwissProt: O75436 Human | P40336 Mouse | Q6AY86 Rat
Alternative names: FLJ12930 HB 58 HB58 Hbeta 58 Hbeta 58 homolog Hbeta58 Hbeta58 homolog hVPS 26 hVPS26 MGC94168 PEP 8A PEP8A Vacuolar protein sorting 26 A Vacuolar protein sorting 26 Vacuolar protein sorting 26 homolog A Vacuolar protein sorting 26 homolog A S. pombe Vacuolar protein sorting 26 homolog A yeast Vacuolar protein sorting 26 yeast Vacuolar protein sorting 26 yeast homolog Vacuolar protein sorting 26A Vacuolar protein sorting associated protein 26A Vacuolar protein sorting-associated protein 26A Vesicle protein sorting 26 Vesicle protein sorting 26A VP26A_HUMAN VPS 26 VPS 26A VPS26A
Images
ET7107-21_1.jpg Fig1: Western blot analysis of VPS26 on different lysates with Rabbit anti-VPS26 antibody (ET7107-21) at 1/500 dilution.

Lane 1: Human liver tissue lysate (20 µg/Lane)
Lane 2: Hela cell lysate (10 µg/Lane)

Predicted band size: 38 kDa
Observed band size: 38 kDa

Exposure time: 2 minutes;

12% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7107-21) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.
ET7107-21_2.jpg Fig2: Immunohistochemical analysis of paraffin-embedded rat kidney tissue using anti-VPS26 antibody. Counter stained with hematoxylin.
ET7107-21_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-VPS26 antibody. Counter stained with hematoxylin.
ET7107-21_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-VPS26 antibody. Counter stained with hematoxylin.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.