Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
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Species reactivity: | Human, Mouse, Rat |
Applications: | WB, IHC-P, IP |
Clonality: | Monoclonal |
Clone number: | JB93-41 |
Form: | Liquid |
Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
Concentration: | 1ug/ul |
Purification: | Protein A affinity purified. |
Molecular weight: | Predicted band size: 38 kDa |
Isotype: | IgG |
Immunogen: | Recombinant protein within Human VPS26 aa 210-327 / 327. |
Positive control: | Human liver tissue lysate, Hela cell lysate, rat kidney tissue, human tonsil tissue, mouse testis tissue. |
Subcellular location: | Endosome. Cytoplasm. |
Recommended Dilutions:
WB IHC-P IP |
1:500-1:2,000 1:50-1:200 1:10-1:50 |
Uniprot #: | SwissProt: O75436 Human | P40336 Mouse | Q6AY86 Rat |
Alternative names: | FLJ12930 HB 58 HB58 Hbeta 58 Hbeta 58 homolog Hbeta58 Hbeta58 homolog hVPS 26 hVPS26 MGC94168 PEP 8A PEP8A Vacuolar protein sorting 26 A Vacuolar protein sorting 26 Vacuolar protein sorting 26 homolog A Vacuolar protein sorting 26 homolog A S. pombe Vacuolar protein sorting 26 homolog A yeast Vacuolar protein sorting 26 yeast Vacuolar protein sorting 26 yeast homolog Vacuolar protein sorting 26A Vacuolar protein sorting associated protein 26A Vacuolar protein sorting-associated protein 26A Vesicle protein sorting 26 Vesicle protein sorting 26A VP26A_HUMAN VPS 26 VPS 26A VPS26A |
Fig1:
Western blot analysis of VPS26 on different lysates with Rabbit anti-VPS26 antibody (ET7107-21) at 1/500 dilution. Lane 1: Human liver tissue lysate (20 µg/Lane) Lane 2: Hela cell lysate (10 µg/Lane) Predicted band size: 38 kDa Observed band size: 38 kDa Exposure time: 2 minutes; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7107-21) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
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Fig2: Immunohistochemical analysis of paraffin-embedded rat kidney tissue using anti-VPS26 antibody. Counter stained with hematoxylin. | |
Fig3: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-VPS26 antibody. Counter stained with hematoxylin. | |
Fig4: Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-VPS26 antibody. Counter stained with hematoxylin. |