VPS26 Recombinant Rabbit Monoclonal Antibody [JB93-41]
cat.: ET7107-21
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P, IP |
| Clonality: | Monoclonal |
| Clone number: | JB93-41 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 38 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human VPS26 aa 210-327 / 327. |
| Positive control: | Human liver tissue lysate, Hela cell lysate, rat kidney tissue, human tonsil tissue, mouse testis tissue. |
| Subcellular location: | Endosome. Cytoplasm. |
| Recommended Dilutions:
WB IHC-P IP |
1:1,000-1:2,000 1:50-1:200 1:10-1:50 |
| Uniprot #: | SwissProt: O75436 Human | P40336 Mouse | Q6AY86 Rat |
| Alternative names: | FLJ12930 HB 58 HB58 Hbeta 58 Hbeta 58 homolog Hbeta58 Hbeta58 homolog hVPS 26 hVPS26 MGC94168 PEP 8A PEP8A Vacuolar protein sorting 26 A Vacuolar protein sorting 26 Vacuolar protein sorting 26 homolog A Vacuolar protein sorting 26 homolog A S. pombe Vacuolar protein sorting 26 homolog A yeast Vacuolar protein sorting 26 yeast Vacuolar protein sorting 26 yeast homolog Vacuolar protein sorting 26A Vacuolar protein sorting associated protein 26A Vacuolar protein sorting-associated protein 26A Vesicle protein sorting 26 Vesicle protein sorting 26A VP26A_HUMAN VPS 26 VPS 26A VPS26A |
Images
|
Fig1:
Western blot analysis of VPS26 on different lysates with Rabbit anti-VPS26 antibody (ET7107-21) at 1/500 dilution. Lane 1: Human liver tissue lysate (20 µg/Lane) Lane 2: Hela cell lysate (10 µg/Lane) Predicted band size: 38 kDa Observed band size: 38 kDa Exposure time: 2 minutes; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7107-21) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Western blot analysis of VPS26 on different lysates with Rabbit anti-VPS26 antibody (ET7107-21) at 1/1,000 dilution. Lane 1: HAP1-parental cell lysate Lane 2: HAP1-VPS26 KD cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 38 kDa Observed band size: 38 kDa Exposure time: 10 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7107-21) at 1/1,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig3: Immunohistochemical analysis of paraffin-embedded rat kidney tissue using anti-VPS26 antibody. Counter stained with hematoxylin. |
|
Fig4: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-VPS26 antibody. Counter stained with hematoxylin. |
|
Fig5: Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-VPS26 antibody. Counter stained with hematoxylin. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.