Proteasome 20S LMP2 Recombinant Rabbit Monoclonal Antibody [JB35-07]
cat.: ET7107-24
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB, IHC-P, IP |
| Clonality: | Monoclonal |
| Clone number: | JB35-07 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 23 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human Proteasome 20S LMP2 aa 85-219 / 219. |
| Positive control: | Mouse spleen tissue lysate, U937 cell lysate, mouse colon tissue lysate, human spleen tissue. |
| Subcellular location: | Cytoplasm. Nucleus. |
| Recommended Dilutions:
WB IHC-P IP |
1:500-1:1,000 1:50-1:200 Use at an assay dependent concentration. |
| Uniprot #: | SwissProt: P28065 Human | P28076 Mouse |
| Alternative names: | Beta1i Large multifunctional peptidase 2 Large multifunctional protease 2 LMP 2 LMP2 Low molecular mass protein 2 Macropain chain 7 MGC70470 Multicatalytic endopeptidase complex chain 7 OTTHUMP00000062982 Proteasome (prosome macropain) subunit beta type 9 proteasome (prosome, macropain) subunit, beta type, 9 (large multifunctional peptidase 2) Proteasome beta 9 subunit Proteasome catalytic subunit 1i Proteasome chain 7 Proteasome related gene 2 Proteasome subunit beta 6i Proteasome subunit beta type 9 Proteasome subunit beta type-9 Proteasome subunit beta-1i PSB9_HUMAN PSMB 9 PSMB6i PSMB9 Really interesting new gene 12 protein RING 12 RING12 RING12 protein |
Images
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Fig1:
Western blot analysis of Proteasome 20S LMP2 on different lysates using anti-Proteasome 20S LMP2 antibody at 1/500 dilution. Positive control: Lane 1: Mouse spleen tissue lysate Lane 2: U937 cell lysate Lane 3: Mouse colon tissue lysate |
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Fig2:
Immunohistochemical analysis of paraffin-embedded human spleen tissue with Rabbit anti-Proteasome 20S LMP2 antibody (ET7107-24) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7107-24) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.