Ikaros Recombinant Rabbit Monoclonal Antibody [JB50-38]
cat.: ET7107-25
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P, FC |
| Clonality: | Monoclonal |
| Clone number: | JB50-38 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 58 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human Ikaros aa 261-353 / 519. |
| Positive control: | Raji cell lysate, Daudi cell lysate, rat colon tissue, human spleen tissue, mouse spleen tissue, human tonsil tissue, Daudi. |
| Subcellular location: | Nucleus. |
| Recommended Dilutions:
WB IHC-P FC |
1:500-1:2,000 1:50-1:1,000 1:50-1:100 |
| Uniprot #: | SwissProt: Q13422 Human | Q03267 Mouse Entrez Gene: 305501 Rat |
| Alternative names: | CLL associated antigen KW 6 DNA-binding protein Ikaros hIk 1 Hs.54452 IK1 Ikaros (zinc finger protein) IKAROS IKAROS family zinc finger 1 (Ikaros) Ikaros family zinc finger protein 1 Ikzf1 IKZF1_HUMAN LYF1 Lymphoid transcription factor LyF-1 PRO0758 Zinc finger protein subfamily 1A 1 (Ikaros) Zinc finger protein subfamily 1A 1 Zinc finger protein, subfamily 1A, member 1 ZNFN1A1 |
Images
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Fig1:
Western blot analysis of Ikaros on different lysates with Rabbit anti-Ikaros antibody (ET7107-25) at 1/1,000 dilution. Lane 1: Raji cell lysate Lane 2: Daudi cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 58 kDa Observed band size: 50-70 kDa Exposure time: 30 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7107-25) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunohistochemical analysis of paraffin-embedded rat colon tissue with Rabbit anti-Ikaros antibody (ET7107-25) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7107-25) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3: Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-Ikaros antibody. Counter stained with hematoxylin. |
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Fig4: Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using anti-Ikaros antibody. Counter stained with hematoxylin. |
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Fig5: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Ikaros antibody. Counter stained with hematoxylin. |
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Fig6: Flow cytometric analysis of Daudi cells with Ikaros antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.