Anti-CRM1 antibody [JB35-22]
cat.: ET7107-27
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, ICC, IF, IHC-P, FC
Clonality: Monoclonal
Clone number: JB35-22
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1 mg/mL.
Purification: Protein A affinity purified.
Molecular weight: 123 kDa
Isotype: IgG
Immunogen: Recombinant protein within C-terminal Human CRM1 .
Positive control: 293T, Hela, A431, LOVO, SiHa, K562, rat skeletal muscle tissue, human lung cancer tissue, human tonsil tissue, mouse skeletal muscle tissue.
Subcellular location: Nucleus. Cytoplasm.
Recommended Dilutions:
  WB
  ICC
  IHC-P
  FC

1:500
1:50-1:200
1:50-1:100
1:50-1:100
Uniprot #: SwissProt: O14980 Human | Q6P5F9 Mouse | Q80U96 Rat
Alternative names: Chromosome region maintenance 1 protein homolog CRM 1 CRM1 homolog DKFZp686B1823 emb Exp 1 Exp1 Exportin 1 Exportin-1 Exportin1 XPO 1 xpo1 XPO1_HUMAN
Images
ET7107-27_1.jpg Fig1: Western blot analysis of CRM1 on 293T and Hela cell lysates using anti-CRM1 antibody at 1/500 dilution.
ET7107-27_2.jpg Fig2: ICC staining CRM1 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ET7107-27_3.jpg Fig3: ICC staining CRM1 in SiHa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ET7107-27_4.jpg Fig4: ICC staining CRM1 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ET7107-27_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-CRM1 antibody. Counter stained with hematoxylin.
ET7107-27_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-CRM1 antibody. Counter stained with hematoxylin.
ET7107-27_7.jpg Fig7: Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue using anti-CRM1 antibody. Counter stained with hematoxylin.
ET7107-27_8.jpg Fig8: Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue using anti-CRM1 antibody. Counter stained with hematoxylin.
ET7107-27_9.jpg Fig9: Flow cytometric analysis of K562 cells with CRM1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.