Nesprin 1 Recombinant Rabbit Monoclonal Antibody [JB35-26]
cat.: ET7107-28
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, IF-Tissue, IHC-P, FC |
| Clonality: | Monoclonal |
| Clone number: | JB35-26 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 1,011 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human Nesprin 1 aa 8,241-8,290 / 8,797. |
| Positive control: | A549, mouse spleen tissue lysate, C2C12, HUVEC, rat brain tissue, human tonsil tissue, human colon cancer tissue, human kidney tissue, mouse fallopian tubes tissue, Daudi. |
| Subcellular location: | Cytoskeleton. Nucleus. |
| Recommended Dilutions:
WB IF-Cell IHC-P FC |
1:500-1:2,000 1:200-1:1,000 1:50-1:200 1:50-1:100 |
| Uniprot #: | SwissProt: Q8NF91 Human | Q6ZWR6 Mouse Entrez Gene: 499010 Rat |
| Alternative names: | 8B ARCA1 C6orf98 CPG2 CPG2 full length dJ45H2.2 EDMD4 Enaptin Myne-1 MYNE1 Myocyte nuclear envelope protein 1 Nesp1 Nesprin-1 Nuclear envelope spectrin repeat protein 1 SCAR8 Spectrin repeat containing nuclear envelope 1 Synaptic nuclear envelope protein 1 Synaptic nuclei expressed gene 1 Syne-1 SYNE1 SYNE1_HUMAN SYNE1B |
Images
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Fig1:
Western blot analysis of Nesprin 1 on different lysates using anti-Nesprin 1 antibody at 1/500 dilution. Positive control: Lane 1: A549 cell lysate Lane 2: Mouse spleen tissue lysate |
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Fig2: ICC staining Nesprin 1 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig3: ICC staining Nesprin 1 in C2C12 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig4: ICC staining Nesprin 1 in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig5: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-Nesprin 1 antibody. Counter stained with hematoxylin. |
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Fig6:
Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-Nesprin 1 antibody (ET7107-28) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7107-28) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig7: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-Nesprin 1 antibody. Counter stained with hematoxylin. |
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Fig8: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Nesprin 1 antibody. Counter stained with hematoxylin. |
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Fig9: Immunohistochemical analysis of paraffin-embedded mouse fallopian tubes tissue using anti-Nesprin 1 antibody. Counter stained with hematoxylin. |
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Fig10: Flow cytometric analysis of Daudi cells with Nesprin 1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.