PCK2 Recombinant Rabbit Monoclonal Antibody [JB52-39]
cat.: ET7107-29
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IF-Cell, IF-Tissue, IHC-P, FC
Clonality: Monoclonal
Clone number: JB52-39
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 71 kDa
Isotype: IgG
Immunogen: Recombinant protein within C-terminal Human PCK2 .
Positive control: Mouse colon tissue lysate, MCF-7 cell lysate, human kidney tissue lysate, MCF7, Neuro-2a, mouse colon tissue, human kidney tissue, human liver tissue, rat kidney tissue, human colon cancer tissue.
Subcellular location: Mitochondrion.
Recommended Dilutions:
  WB
  IF-Cell
  IHC-P
  FC
1:1,000-1:2,000
1:100
1:50-1:200
1:50-1:100
Uniprot #: SwissProt: Q16822 Human | Q8BH04 Mouse
Entrez Gene: 361042 Rat
Alternative names: EC 4.1.1.32 GTP mitochondrial precursor HGNC:8725 mitochondrial Mitochondrial phosphoenolpyruvate carboxykinase 2 OTTHUMP00000164700 PCK2 PCKGM_HUMAN PE PEP carboxykinase PEPCK PEPCK deficiency mitochondrial PEPCK M PEPCK-M PEPCK2 Phosphoenolpyruvate carboxykinase [GTP] Phosphoenolpyruvate carboxykinase 2 (mitochondrial) Phosphoenolpyruvate carboxykinase 2 mitochondrial Phosphoenolpyruvate carboxylase Phosphopyruvate carboxylase
Images
ET7107-29_1.jpg Fig1: Western blot analysis of PCK2 on different lysates with Rabbit anti-PCK2 antibody (ET7107-29) at 1/2,000 dilution.

Lane 1: MCF7 cell lysate (20 µg/Lane)
Lane 2: HeLa cell lysate (20 µg/Lane)
Lane 3: Neuro-2a cell lysate (20 µg/Lane)
Lane 4: C6 cell lysate (20 µg/Lane)
Lane 5: Mouse brain tissue lysate (40 µg/Lane)
Lane 6: Rat brain tissue lysate (40 µg/Lane)

Predicted band size: 71 kDa
Observed band size: 67 kDa

Exposure time: 20 seconds; ECL: K1801;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7107-29) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
ET7107-29_2.jpg Fig2: Western blot analysis of PCK2 on different lysates with Rabbit anti-PCK2 antibody (ET7107-29) at 1/1,000 dilution.

Lane 1: HAP1-parental cell lysate
Lane 2: HAP1-PCK2 KD cell lysate

Lysates/proteins at 10 µg/Lane.

Predicted band size: 71 kDa
Observed band size: 67 kDa

Exposure time: 21 seconds; ECL: K1801;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7107-29) at 1/1,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
ET7107-29_3.jpg Fig3: Immunocytochemistry analysis of MCF7 cells labeling PCK2 with Rabbit anti-PCK2 antibody (ET7107-29) at 1/100 dilution.

Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-PCK2 antibody (ET7107-29) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Counterstained with Mitotracker. Nuclear DNA was labelled in blue with DAPI.
ET7107-29_4.jpg Fig4: Immunocytochemistry analysis of Neuro-2a cells labeling PCK2 with Rabbit anti-PCK2 antibody (ET7107-29) at 1/100 dilution.

Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-PCK2 antibody (ET7107-29) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Counterstained with Mitotracker. Nuclear DNA was labelled in blue with DAPI.
ET7107-29_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-PCK2 antibody. Counter stained with hematoxylin.
ET7107-29_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-PCK2 antibody. Counter stained with hematoxylin.
ET7107-29_7.jpg Fig7: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-PCK2 antibody. Counter stained with hematoxylin.
ET7107-29_8.jpg Fig8: Flow cytometric analysis of MCF-7 cells with PCK2 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.