PCK2 Recombinant Rabbit Monoclonal Antibody [JB52-39]
cat.: ET7107-29
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, IF-Tissue, IHC-P, FC |
| Clonality: | Monoclonal |
| Clone number: | JB52-39 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 71 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within C-terminal Human PCK2 . |
| Positive control: | Mouse colon tissue lysate, MCF-7 cell lysate, human kidney tissue lysate, HepG2, MCF-7, mouse colon tissue, human kidney tissue, human liver tissue, rat kidney tissue, human colon cancer tissue. |
| Subcellular location: | Mitochondrion. |
| Recommended Dilutions:
WB IF-Cell IHC-P FC |
1:500-1:1,000 1:50-1:100 1:50-1:200 1:50-1:100 |
| Uniprot #: | SwissProt: Q16822 Human | Q8BH04 Mouse Entrez Gene: 361042 Rat |
| Alternative names: | EC 4.1.1.32 GTP mitochondrial precursor HGNC:8725 mitochondrial Mitochondrial phosphoenolpyruvate carboxykinase 2 OTTHUMP00000164700 PCK2 PCKGM_HUMAN PE PEP carboxykinase PEPCK PEPCK deficiency mitochondrial PEPCK M PEPCK-M PEPCK2 Phosphoenolpyruvate carboxykinase [GTP] Phosphoenolpyruvate carboxykinase 2 (mitochondrial) Phosphoenolpyruvate carboxykinase 2 mitochondrial Phosphoenolpyruvate carboxylase Phosphopyruvate carboxylase |
Images
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Fig1:
Western blot analysis of PCK2 on different lysates with Rabbit anti-PCK2 antibody (ET7107-29) at 1/500 dilution. Lane 1: Mouse colon tissue lysate (20 µg/Lane) Lane 2: MCF-7 cell lysate (10 µg/Lane) Lane 3: Human kidney tissue lysate (20 µg/Lane) Predicted band size: 71 kDa Observed band size: 71 kDa Exposure time: 1 minute; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7107-29) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
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Fig2: ICC staining PCK2 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig3: ICC staining PCK2 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig4: Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-PCK2 antibody. Counter stained with hematoxylin. |
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Fig5: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-PCK2 antibody. Counter stained with hematoxylin. |
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Fig6: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-PCK2 antibody. Counter stained with hematoxylin. |
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Fig7: Immunohistochemical analysis of paraffin-embedded rat kidney tissue using anti-PCK2 antibody. Counter stained with hematoxylin. |
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Fig8: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-PCK2 antibody. Counter stained with hematoxylin. |
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Fig9: Flow cytometric analysis of MCF-7 cells with PCK2 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.