HIF-2 alpha Recombinant Rabbit Monoclonal Antibody [JB24-42]
cat.: ET7107-32
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P, FC |
| Clonality: | Monoclonal |
| Clone number: | JB24-42 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 96 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human HIF-2 alpha aa 61-110 / 870. |
| Positive control: | Mouse lung tissue lysate, Siha cell lysate, rat lung tissue, human breast tissue, human placenta tissue, mouse colon tissue, HUVEC. |
| Subcellular location: | Nucleus, Nucleus speckle. |
| Recommended Dilutions:
WB IHC-P FC |
1:500-1:2,000 1:50-1:1,000 1:50-1:100 |
| Uniprot #: | SwissProt: Q99814 Human | P97481 Mouse | Q9JHS1 Rat |
| Alternative names: | Basic helix loop helix PAS protein MOP2 Basic-helix-loop-helix-PAS protein MOP2 bHLHe73 Class E basic helix-loop-helix protein 73 ECYT4 Endothelial PAS domain containing protein 1 Endothelial pas domain protein 1 Endothelial PAS domain-containing protein 1 EPAS 1 EPAS-1 EPAS1 EPAS1_HUMAN HIF 1 alpha like factor HIF 2 alpha HIF-1-alpha-like factor HIF-2-alpha HIF2-alpha HIF2A HLF Hypoxia inducible factor 2 alpha Hypoxia inducible factor 2 alpha subunit Hypoxia-inducible factor 2-alpha Member of PAS protein 2 Member of pas superfamily 2 MOP 2 MOP2 PAS domain-containing protein 2 PASD2 |
Images
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Fig1:
Western blot analysis of HIF-2 alpha on different lysates with Rabbit anti-HIF-2 alpha antibody (ET7107-32) at 1/500 dilution. Lane 1: Mouse lung tissue lysate (20 µg/Lane) Lane 2: Siha cell lysate (10 µg/Lane) Predicted band size: 96 kDa Observed band size: 110 kDa Exposure time: 1 minute; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7107-32) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
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Fig2: Immunohistochemical analysis of paraffin-embedded rat lung tissue using anti-HIF-2 alpha antibody. Counter stained with hematoxylin. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded human breast tissue with Rabbit anti-HIF-2 alpha antibody (ET7107-32) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7107-32) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4: Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-HIF-2 alpha antibody. Counter stained with hematoxylin. |
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Fig5: Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-HIF-2 alpha antibody. Counter stained with hematoxylin. |
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Fig6: Flow cytometric analysis of HUVEC cells with HIF-2 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.