p53 Recombinant Rabbit Monoclonal Antibody [JB42-26]
cat.: ET7107-33
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IHC-P, FC, IP, IF-Cell, IF-Tissue |
| Clonality: | Monoclonal |
| Clone number: | JB42-26 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 44 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human p53 aa 344-393 / 393. |
| Positive control: | A431 cell lysate, 293 cell lysate, human stomach carcinoma tissue, human colon carcinoma tissue, Daudi. |
| Subcellular location: | Cytoplasm. Nucles. |
| Recommended Dilutions:
WB IHC-P FC IF-Cell |
1:500-1:2,000 1:50-1:100 1:50-1:100 1:100-1:500 |
| Uniprot #: | SwissProt: P04637 Human |
| Alternative names: | Antigen NY-CO-13 BCC7 Cellular tumor antigen p53 LFS1 Mutant tumor protein 53 p53 tumor suppressor P53_HUMAN Phosphoprotein p53 Tp53 Transformation related protein 53 TRP53 Tumor protein p53 Tumor suppressor p53 |
Images
|
Fig1:
Western blot analysis of p53 on different lysates with Rabbit anti-p53 antibody (ET7107-33) at 1/500 dilution. Lane 1: A431 cell lysate Lane 2: 293 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 44 kDa Observed band size: 53 kDa Exposure time: 2 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7107-33) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
|
Fig2: Immunohistochemical analysis of paraffin-embedded human stomach carcinoma tissue using anti-p53 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7107-33, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig3: Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using anti-p53 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7107-33, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig4: Flow cytometric analysis of p53 was done on Daudi cells. The cells were fixed, permeabilized and stained with the primary antibody (ET7107-33, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.