Cytochrome P450 Reductase Recombinant Rabbit Monoclonal Antibody [JB54-31]
cat.: ET7107-35
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IF-Cell, IF-Tissue, IHC-P, FC
Clonality: Monoclonal
Clone number: JB54-31
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 77 kDa
Isotype: IgG
Immunogen: Recombinant protein within Human Cytochrome P450 Reductase aa 80-220 / 677.
Positive control: A549 cell lysate, mouse lung tissue lysate, HepG2, LOVO, A549, human liver tissue, mouse testis tissue, rat brain tissue, human lung carcinoma tissue.
Subcellular location: Endoplasmic reticulum.
Recommended Dilutions:
  WB
  IF-Cell
  IHC-P
  FC
1:500-1:1,000
1:50-1:200
1:50-1:200
1:50-1:100
Uniprot #: SwissProt: P16435 Human | P37040 Mouse | P00388 Rat
Alternative names: CPR CYPOR Cytochrome p450 oxidoreductase DKFZp686G04235 FLJ26468 NADPH Cytochrome P450 Reductase NADPH dependent cytochrome P450 reductase NADPH--cytochrome P450 reductase NCPR_HUMAN P450 (cytochrome) oxidoreductase P450 Cytochrome Oxidoreductase P450R por
Images
ET7107-35_1.jpg Fig1: Western blot analysis of Cytochrome P450 Reductase on different lysates with Rabbit anti-Cytochrome P450 Reductase antibody (ET7107-35) at 1/500 dilution.

Lane 1: A549 cell lysate (10 µg/Lane)
Lane 2: Mouse lung tissue lysate (20 µg/Lane)

Predicted band size: 77 kDa
Observed band size: 77 kDa

Exposure time: 2 minutes;

8% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7107-35) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.
ET7107-35_2.jpg Fig2: ICC staining of Cytochrome P450 Reductase in HepG2 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET7107-35, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ET7107-35_3.jpg Fig3: ICC staining of Cytochrome P450 Reductase in LOVO cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET7107-35, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ET7107-35_4.jpg Fig4: ICC staining of Cytochrome P450 Reductase in A549 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET7107-35, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ET7107-35_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Cytochrome P450 Reductase antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7107-35, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET7107-35_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-Cytochrome P450 Reductase antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7107-35, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET7107-35_7.jpg Fig7: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-Cytochrome P450 Reductase antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7107-35, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET7107-35_8.jpg Fig8: Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using anti-Cytochrome P450 Reductase antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7107-35, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET7107-35_9.jpg Fig9: Flow cytometric analysis of Cytochrome P450 Reductase was done on HepG2 cells. The cells were fixed, permeabilized and stained with the primary antibody (ET7107-35, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
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