Furin Recombinant Rabbit Monoclonal Antibody [JB35-53]
cat.: ET7107-37
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IHC-P
Clonality: Monoclonal
Clone number: JB35-53
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 87 kDa
Isotype: IgG
Immunogen: Recombinant protein within Human Furin aa 261-310 / 794.
Positive control: 293T cell lysate, A431 cell lysate, C2C12 cell lysate, PC-12 cell lysate, human liver tissue, human colon tissue, human placenta tissue, mouse brain tissue.
Subcellular location: Secreted. Plasma membrane. Golgi apparatus. Endosome.
Recommended Dilutions:
  WB
  IHC-P
1:2,000
1:50-1:100
Uniprot #: SwissProt: P09958 Human | P23188 Mouse | P23377 Rat
Alternative names: Dibasic processing enzyme Dibasic-processing enzyme FES upstream region FUR FURIN Furin membrane associated receptor protein FURIN_HUMAN PACE Paired basic amino acid residue cleaving enzyme Paired basic amino acid residue-cleaving enzyme PCSK3 Proprotein convertase subtilisin/kexin type 3 SPC1
Images
ET7107-37_1.jpg Fig1: All lanes: Western blot analysis of FURIN with anti-Furin antibody [JB35-53] (ET7107-37) at 1:500 dilution.

Lane 1: Wild-type Hela whole cell lysate.
Lane 2: FURIN knockdown Hela whole cell lysate.

ET7107-37 was shown to specifically react with FURIN in wild-type Hela cells. Weakened band was observed when FURIN knockdown samples were tested. Wild-type and FURIN knockdown samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary Anti-FURIN antibody (ET7107-37, 1/500) and Anti-HSP90 antibody (ET1605-56, 1/10,000) were used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG H&L (HRP) Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.

Cell lysate was provided by Ubigene Biosciences (Ubigene Biosciences Co., Ltd., Guangzhou, China).
ET7107-37_2.jpg Fig2: Western blot analysis of Furin on different lysates with Rabbit anti-Furin antibody (ET7107-37) at 1/2,000 dilution.

Lane 1: 293T cell lysate
Lane 2: A431 cell lysate
Lane 3: Ramos cell lysate (low expression)
Lane 4: C2C12 cell lysate
Lane 5: PC-12 cell lysate

Lysates/proteins at 20 µg/Lane.

Predicted band size: 87 kDa
Observed band size: 75 kDa

Exposure time: 3 minutes; ECL: K1802;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7107-37) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
ET7107-37_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Furin antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7107-37, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET7107-37_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-Furin antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7107-37, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET7107-37_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-Furin antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7107-37, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET7107-37_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-Furin antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7107-37, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.