| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P, FC |
| Clonality: | Monoclonal |
| Clone number: | JB57-37 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 45 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human Calsequestrin 1 aa 80-220 / 396. |
| Positive control: | Rat skeletal muscle tissue, mouse skeletal muscle tissue, human fetal skeletal muscle tissue, C2C12. |
| Subcellular location: | Endoplasmic reticulum. Mitochondrion. |
| Recommended Dilutions:
WB IHC-P FC |
1:500 1:50-1:200 1:50-1:100 |
| Uniprot #: | SwissProt: P31415 Human | O09165 Mouse | P19633 Rat |
| Alternative names: | Calmitin Calmitine Calsequestrin 1 (fast twitch, skeletal muscle) Calsequestrin Calsequestrin, cell Calsequestrin, skeletal muscle isoform Calsequestrin-1 CASQ Casq1 CASQ1_HUMAN PDIB1 skeletal muscle isoform |
|
Fig1: Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue using anti-Calsequestrin 1 antibody. Counter stained with hematoxylin. |
|
Fig2: Immunohistochemical analysis of paraffin-embedded human fetal skeletal muscle tissue using anti-Calsequestrin 1 antibody. Counter stained with hematoxylin. |
|
Fig3: Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue using anti-Calsequestrin 1 antibody. Counter stained with hematoxylin. |
|
Fig4: Flow cytometric analysis of C2C12 cells with Calsequestrin 1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody. |