TGF alpha Recombinant Rabbit Monoclonal Antibody [JB58-36]
cat.: ET7107-40
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IF-Tissue, IHC-P, IP |
| Clonality: | Monoclonal |
| Clone number: | JB58-36 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 17 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human TGF alpha aa 1-160 / 160. |
| Positive control: | MCF7 cell lysate (hot lysis), PANC-1 cell lysate (hot lysis), human colon cancer tissue. |
| Subcellular location: | Plasma membrane. Extracellular region. Secreted. |
| Recommended Dilutions:
WB IF-Tissue IHC-P IP |
1:500 1:200 1:4,000 1:50 |
| Uniprot #: | SwissProt: P01135 Human |
| Alternative names: | EGF like TGF EGF-like TGF ETGF TFGA TGF A TGF type 1 TGF-alpha Tgfa TGFA_HUMAN Transforming growth factor alpha Transforming growth factor alpha precursor Wa1 Waved 1 |
Images
|
Fig1:
Western blot analysis of TGF alpha on different lysates with Rabbit anti-TGF alpha antibody (ET7107-40) at 1/500 dilution. Lane 1: MCF7 cell lysate (RIPA lysis) Lane 2: MCF7 cell lysate (hot lysis) Lane 3: PANC-1 cell lysate (RIPA lysis) Lane 4: PANC-1 cell lysate (hot lysis) Lysates/proteins at 20 µg/Lane. Predicted band size: 17 kDa Observed band size: 17 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7107-40) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Application: Immunohistochemistry (IHC-P) Species: Mouse Tissue: Colon cancer Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95℃. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: ET7107-40, 1/4,000, 1 hour at room temperature. Secondary antibody: HA1119, 20 minutes at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.