TGF alpha Recombinant Rabbit Monoclonal Antibody [JB58-36]
cat.: ET7107-40
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IF-Cell, IF-Tissue, IHC-P, IP |
| Clonality: | Monoclonal |
| Clone number: | JB58-36 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 17 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human TGF alpha aa 1-160 / 160. |
| Positive control: | MCF7 cell lysate (hot lysis), PANC-1 cell lysate (hot lysis), A431, A549, PC-3M, human kidney tissue, human tonsil tissue, human liver tissue, human colon cancer tissue. |
| Subcellular location: | Plasma membrane. Extracellular region. Secreted. |
| Recommended Dilutions:
WB IF-Cell IHC-P IP |
1:500 1:50-1:200 1:50-1:500 1:50 |
| Uniprot #: | SwissProt: P01135 Human |
| Alternative names: | EGF like TGF EGF-like TGF ETGF TFGA TGF A TGF type 1 TGF-alpha Tgfa TGFA_HUMAN Transforming growth factor alpha Transforming growth factor alpha precursor Wa1 Waved 1 |
Images
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Fig1:
Western blot analysis of TGF alpha on different lysates with Rabbit anti-TGF alpha antibody (ET7107-40) at 1/500 dilution. Lane 1: MCF7 cell lysate (RIPA lysis) Lane 2: MCF7 cell lysate (hot lysis) Lane 3: PANC-1 cell lysate (RIPA lysis) Lane 4: PANC-1 cell lysate (hot lysis) Lysates/proteins at 20 µg/Lane. Predicted band size: 17 kDa Observed band size: 17 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7107-40) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature. |
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Fig2: ICC staining TGF alpha in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig3: ICC staining TGF alpha in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig4: ICC staining TGF alpha in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig5: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-TGF alpha antibody. Counter stained with hematoxylin. |
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Fig6: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-TGF alpha antibody. Counter stained with hematoxylin. |
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Fig7: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-TGF alpha antibody. Counter stained with hematoxylin. |
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Fig8: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-TGF alpha antibody. Counter stained with hematoxylin. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.