TGF alpha Recombinant Rabbit Monoclonal Antibody [JB58-36]
cat.: ET7107-40
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IF-Tissue, IHC-P, IP |
| Clonality: | Monoclonal |
| Clone number: | JB58-36 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 17 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human TGF alpha aa 1-160 / 160. |
| Positive control: | MCF7 cell lysate (hot lysis), PANC-1 cell lysate (hot lysis), human liver tissue, human kidney tissue, human tonsil tissue, human colon cancer tissue. |
| Subcellular location: | Plasma membrane. Extracellular region. Secreted. |
| Recommended Dilutions:
WB IF-Tissue IHC-P IP |
1:500 1:200 1:200-1:1,000 1:50 |
| Uniprot #: | SwissProt: P01135 Human |
| Alternative names: | EGF like TGF EGF-like TGF ETGF TFGA TGF A TGF type 1 TGF-alpha Tgfa TGFA_HUMAN Transforming growth factor alpha Transforming growth factor alpha precursor Wa1 Waved 1 |
Images
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Fig1:
Western blot analysis of TGF alpha on different lysates with Rabbit anti-TGF alpha antibody (ET7107-40) at 1/500 dilution. Lane 1: MCF7 cell lysate (RIPA lysis) Lane 2: MCF7 cell lysate (hot lysis) Lane 3: PANC-1 cell lysate (RIPA lysis) Lane 4: PANC-1 cell lysate (hot lysis) Lysates/proteins at 20 µg/Lane. Predicted band size: 17 kDa Observed band size: 17 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7107-40) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-TGF alpha antibody (ET7107-40) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7107-40) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-TGF alpha antibody. Counter stained with hematoxylin. |
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Fig4: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-TGF alpha antibody. Counter stained with hematoxylin. |
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Fig5: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-TGF alpha antibody. Counter stained with hematoxylin. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.