DDAH2 Recombinant Rabbit Monoclonal Antibody [JB59-33]
cat.: ET7107-41
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IP
Clonality: Monoclonal
Clone number: JB59-33
Form: Liquid
Storage condition: Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 30 kDa
Isotype: IgG
Immunogen: Recombinant protein within Human DDAH2 aa 1-125 / 285.
Positive control: MCF7 cell lysate, mouse lung tissue lysate, rat lung tissue lysate.
Subcellular location: Cytoplasm. Mitochondrion.
Recommended Dilutions:
  WB
  IP

1:2,000-1:5,000
1:10-:50
Uniprot #: SwissProt: O95865 Human | Q99LD8 Mouse | Q6MG60 Rat
Alternative names: DDAH DDAH II DDAH-2 DDAH2 DDAH2_HUMAN DDAHII Dimethylargininase 2 Dimethylargininase-2 Dimethylarginine dimethylaminohydrolase 2 Dimethylarginine dimethylaminohydrolase II G6a N(G),N(G)-dimethylarginine dimethylaminohydrolase 2 NG dimethylarginine dimethylamino hydrolase homolog NG30 OTTHUMP00000029307 OTTHUMP00000029310 OTTHUMP00000174488 OTTHUMP00000174489 Protein G6a S phase protein S-phase protein
Images
ET7107-41_1.jpg Fig1: Western blot analysis of DDAH2 on different lysates with Rabbit anti-DDAH2 antibody (ET7107-41) at 1/5,000 dilution.

Lane 1: MCF7 (Human breast cancer cell) cell lysate (15 µg/Lane)
Lane 2: Mouse lung tissue lysate (30 µg/Lane)
Lane 3: Rat lung tissue lysate (30 µg/Lane)

Exposure time: 20 seconds; ECL: K1802

Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: ET7107-41, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature

Predicted band size: 30 kDa
Observed band size: 30 kDa
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.