| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IP |
| Clonality: | Monoclonal |
| Clone number: | JB59-33 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 30 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human DDAH2 aa 1-125 / 285. |
| Positive control: | MCF7 cell lysate, mouse lung tissue lysate, rat lung tissue lysate. |
| Subcellular location: | Cytoplasm. Mitochondrion. |
| Recommended Dilutions:
WB IP |
1:2,000-1:5,000 1:10-:50 |
| Uniprot #: | SwissProt: O95865 Human | Q99LD8 Mouse | Q6MG60 Rat |
| Alternative names: | DDAH DDAH II DDAH-2 DDAH2 DDAH2_HUMAN DDAHII Dimethylargininase 2 Dimethylargininase-2 Dimethylarginine dimethylaminohydrolase 2 Dimethylarginine dimethylaminohydrolase II G6a N(G),N(G)-dimethylarginine dimethylaminohydrolase 2 NG dimethylarginine dimethylamino hydrolase homolog NG30 OTTHUMP00000029307 OTTHUMP00000029310 OTTHUMP00000174488 OTTHUMP00000174489 Protein G6a S phase protein S-phase protein |
|
Fig1:
Western blot analysis of DDAH2 on different lysates with Rabbit anti-DDAH2 antibody (ET7107-41) at 1/5,000 dilution. Lane 1: MCF7 (Human breast cancer cell) cell lysate (15 µg/Lane) Lane 2: Mouse lung tissue lysate (30 µg/Lane) Lane 3: Rat lung tissue lysate (30 µg/Lane) Exposure time: 20 seconds; ECL: K1802 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: ET7107-41, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 30 kDa Observed band size: 30 kDa |