KDM5A Recombinant Rabbit Monoclonal Antibody [JB36-60]
cat.: ET7107-43
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IF-Cell, IF-Tissue, FC |
| Clonality: | Monoclonal |
| Clone number: | JB36-60 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 192 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human KDM5A aa 1,360-1,690 / 1,690. |
| Positive control: | A549 cell lysate, Hela cell lysate, A549, SiHa, SH-SY5Y. |
| Subcellular location: | Nucleus. |
| Recommended Dilutions:
WB IF-Cell FC |
1:1,000-1:2,000 1:50-1:400 1:50-1:100 |
| Uniprot #: | SwissProt: P29375 Human |
| Alternative names: | Histone demethylase JARID1A JARID1A Jumonji/ARID domain containing protein 1A Jumonji/ARID domain-containing protein 1A Kdm5a KDM5A_HUMAN Lysine-specific demethylase 5A RBBP-2 RBBP2 RBP2 Retinoblastoma binding protein 2 Retinoblastoma-binding protein 2 |
Images
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Fig1:
Western blot analysis of KDM5A on different lysates with Rabbit anti-KDM5A antibody (ET7107-43) at 1/1,000 dilution. Lane 1: A549 cell lysate Lane 2: Hela cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 192 kDa Observed band size: 200 kDa Exposure time: 2 minutes; 6% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7107-43) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Western blot analysis of KDM5A on different lysates with Rabbit anti-KDM5A antibody (ET7107-43) at 1/2,000 dilution. Lane 1: HAP1-parental cell lysate Lane 2: HAP1-KDM5A KD cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 192 kDa Observed band size: 200 kDa Exposure time: 180 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7107-43) at 1/2,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig3: ICC staining of KDM5A in A549 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (ET7107-43, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue). |
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Fig4: ICC staining of KDM5A in SiHa cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (ET7107-43, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue). |
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Fig5: Flow cytometric analysis of KDM5A was done on SH-SY5Y cells. The cells were fixed, permeabilized and stained with the primary antibody (ET7107-43, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.