SRC1 Recombinant Rabbit Monoclonal Antibody [JB66-31]
cat.: ET7107-44
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IHC-P, IP, IF-Cell |
| Clonality: | Monoclonal |
| Clone number: | JB66-31 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 157 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human SRC1 aa 380-770 / 1,441. |
| Positive control: | Raji cell lysate, HeLa cell lysate, 293T cell lysate, 293T, human breast cancer tissue. |
| Subcellular location: | Nucleus. |
| Recommended Dilutions:
WB IHC-P IP IF-Cell |
1:500-1:2,000 1:500-1:1,000 Use at an assay dependent concentration. 1:100 |
| Uniprot #: | SwissProt: Q15788 Human |
| Alternative names: | bHLHe74 Class E basic helix-loop-helix protein 74 F SRC 1 Hin 2 protein Hin2 protein MGC129719 MGC129720 mNRC 1 NCoA 1 NCoA-1 Ncoa1 NCOA1_HUMAN Nuclear receptor coactivator 1 Nuclear receptor coactivator protein 1 NY REN 52 antigen Protein Hin 2 Protein Hin-2 Protein Hin2 Renal carcinoma antigen NY REN 52 Renal carcinoma antigen NY-REN-52 RIP 160 RIP160 SRC 1 SRC-1 Steroid receptor coactivator 1 |
Images
|
Fig1:
Western blot analysis of SRC1 on different lysates with Rabbit anti-SRC1 antibody (ET7107-44) at 1/2,000 dilution. Lane 1: Raji cell lysate Lane 2: HeLa cell lysate Lane 3: 293T cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 157 kDa Observed band size: 157 kDa Exposure time: 1 minute; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7107-44) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunocytochemistry analysis of 293T cells labeling SRC1 with Rabbit anti-SRC1 antibody (ET7107-44) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-SRC1 antibody (ET7107-44) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
|
Fig3:
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue with Rabbit anti-SRC1 antibody (ET7107-44) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7107-44) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.