PU.1/Spi1 Recombinant Rabbit Monoclonal Antibody [JB72-35]
cat.: ET7107-49
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IHC-P, FC |
| Clonality: | Monoclonal |
| Clone number: | JB72-35 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 31 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human PU1 / Spi1 aa 1-50 / 270. |
| Positive control: | THP-1 cell lysates, human spleen tissue, human tonsil tissue, K562. |
| Subcellular location: | Cytosol. |
| Recommended Dilutions:
WB IHC-P FC |
1:500-1:1,000 1:50-1:200 1:50-1:100 |
| Uniprot #: | SwissProt: P17947 Human |
| Alternative names: | transcription factor spi1 31 kDa Transforming Protein 31 kDa-transforming protein cb1086 Hematopoietic transcription factor PU.1 OF oncogene spi1 PU.1 SFFV virus-induced murine erythroleukemia oncogene, mouse, homolog of SFPI1 si:by184l24.2 SPI 1 SPI 1 proto oncogene SPI A Spi1 SPI1_HUMAN Spleen focus forming virus (SFFV) proviral integration oncogene spi1 Spleen focus forming virus proviral integration oncogene spi1 Transcription factor PU.1 |
Images
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Fig1: Western blot analysis of PU.1/Spi1 on THP-1 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET7107-49, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:40,000 dilution was used for 1 hour at room temperature. |
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Fig2: Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-PU.1/Spi1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7107-49, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-PU.1/Spi1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7107-49, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4: Flow cytometric analysis of PU.1/Spi1 was done on K562 cells. The cells were fixed, permeabilized and stained with the primary antibody (ET7107-49, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.