Anti-Securin antibody [JB37-37]
cat.: ET7107-50
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human
Applications: WB, ICC, IF, IHC-P, FC, IP
Clonality: Monoclonal
Clone number: JB37-37
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1 mg/mL.
Purification: Protein A purified.
Molecular weight: 22 kDa
Isotype: IgG
Immunogen: Recombinant protein within C-terminal Human Securin .
Positive control: SiHa, A431, HUVEC, PC-3M, human tonsil tissue, human colon cancer tissue.
Subcellular location: Cytoplasm. Nucleus.
Recommended Dilutions:
  WB
  ICC
  IHC-P
  FC

1:500
1:50-1:200
1:50-1:200
1:50-1:100
Uniprot #: O95997 Q6IAL9(Human)
Alternative names: AW555095 antibody C87862 antibody Cut2 antibody EAP 1 antibody EAP1 antibody ESP1 associated protein 1 antibody Esp1-associated protein antibody hPTTG antibody MGC126883 antibody MGC138276 antibody Pds1 antibody Pituitary tumor transforming 1 antibody Pituitary tumor transforming protein 1 antibody Pituitary tumor-transforming 1, isoform CRA_a antibody Pituitary tumor-transforming 1, isoform CRA_b antibody Pituitary tumor-transforming gene 1 antibody Pituitary tumor-transforming gene 1 protein antibody PTTG 1 antibody PTTG antibody PTTG1 antibody PTTG1 protein antibody PTTG1_HUMAN antibody Pttg3 antibody Securin antibody Tumor transforming 1 antibody Tumor transforming protein 1 antibody Tumor-transforming protein 1 antibody TUTR 1 antibody TUTR1 antibody
Images
ET7107-50_1.jpg Fig1: Western blot analysis of Securin on SiHa cell lysate using anti-Securin antibody at 1/500 dilution.
ET7107-50_2.jpg Fig2: ICC staining Securin in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ET7107-50_3.jpg Fig3: ICC staining Securin in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ET7107-50_4.jpg Fig4: ICC staining Securin in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ET7107-50_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Securin antibody. Counter stained with hematoxylin.
ET7107-50_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-Securin antibody. Counter stained with hematoxylin.
ET7107-50_7.jpg Fig7: Flow cytometric analysis of A431 cells with Securin antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.