Anti-Securin antibody [JB37-37]
cat.: ET7107-50
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, ICC, IF, IHC-P, FC, IP |
| Clonality: | Monoclonal |
| Clone number: | JB37-37 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1 mg/mL. |
| Purification: | Protein A purified. |
| Molecular weight: | 22 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within C-terminal Human Securin . |
| Positive control: | SiHa, A431, HUVEC, PC-3M, human tonsil tissue, human colon cancer tissue. |
| Subcellular location: | Cytoplasm. Nucleus. |
| Recommended Dilutions:
WB ICC IHC-P FC |
1:500 1:50-1:200 1:50-1:200 1:50-1:100 |
| Uniprot #: | O95997 Q6IAL9(Human) |
| Alternative names: | AW555095 antibody C87862 antibody Cut2 antibody EAP 1 antibody EAP1 antibody ESP1 associated protein 1 antibody Esp1-associated protein antibody hPTTG antibody MGC126883 antibody MGC138276 antibody Pds1 antibody Pituitary tumor transforming 1 antibody Pituitary tumor transforming protein 1 antibody Pituitary tumor-transforming 1, isoform CRA_a antibody Pituitary tumor-transforming 1, isoform CRA_b antibody Pituitary tumor-transforming gene 1 antibody Pituitary tumor-transforming gene 1 protein antibody PTTG 1 antibody PTTG antibody PTTG1 antibody PTTG1 protein antibody PTTG1_HUMAN antibody Pttg3 antibody Securin antibody Tumor transforming 1 antibody Tumor transforming protein 1 antibody Tumor-transforming protein 1 antibody TUTR 1 antibody TUTR1 antibody |
Images
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Fig1: Western blot analysis of Securin on SiHa cell lysate using anti-Securin antibody at 1/500 dilution. |
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Fig2: ICC staining Securin in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig3: ICC staining Securin in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig4: ICC staining Securin in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig5: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Securin antibody. Counter stained with hematoxylin. |
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Fig6: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-Securin antibody. Counter stained with hematoxylin. |
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Fig7: Flow cytometric analysis of A431 cells with Securin antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.