RPA70 Recombinant Rabbit Monoclonal Antibody [JB75-32]
cat.: ET7107-51
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Rat
Applications: WB, IF-Cell, IF-Tissue, FC
Clonality: Monoclonal
Clone number: JB75-32
Form: Liquid
Storage condition: Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 68 kDa
Isotype: IgG
Immunogen: Synthetic peptide within Human RPA70 aa 22-71 / 616.
Positive control: HeLa cell lysate, HEK-293 cell lysate, U-2 OS cell lysate, MCF7 cell lysate, K-562 cell lysate, PC-12 cell lysates, Daudi, C6.
Subcellular location: Nucleus.
Recommended Dilutions:
  WB
  IF-Cell
  FC
1:1,000-1:5,000
1:100-1:500
1:1,000
Uniprot #: SwissProt: P27694 Human
Entrez Gene: 287524 Rat
Alternative names: Dmrpa1 Drosophila Replication Protein A DRPA HSSB Human single stranded DNA binding protein MST075 MSTP075 p70 REPA1 Replication factor A Replication factor A protein 1 Replication protein A 70 kDa DNA-binding subunit Replication protein A 70kDa DNA binding subunit Replication protein A1 70kDa Replication protein A1 RF A RF-A protein 1 RFA RFA1_HUMAN RP A RP-A p70 RPA 70 RPA rpa1 Single stranded binding protein 70 Single-stranded DNA-binding protein
Images
ET7107-51_1.jpg Fig1: Western blot analysis of RPA70 on different lysates with Rabbit anti-RPA70 antibody (ET7107-51) at 1/1,000 dilution.

Lane 1: HeLa cell lysate
Lane 2: HEK-293 cell lysate
Lane 3: U-2 OS cell lysate
Lane 4: MCF7 cell lysate
Lane 5: K-562 cell lysate

Lysates/proteins at 20 µg/Lane.

Predicted band size: 68 kDa
Observed band size: 68 kDa

Exposure time: 25 seconds; ECL: K1801;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7107-51) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
ET7107-51_2.jpg Fig2: Western blot analysis of RPA70 on PC-12 cell lysates with Rabbit anti-RPA70 antibody (ET7107-51) at 1/5,000 dilution.

Lysates/proteins at 20 µg/Lane.

Predicted band size: 68 kDa
Observed band size: 68 kDa

Exposure time: 16 seconds; ECL: K1801;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7107-51) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
ET7107-51_3.jpg Fig3: Immunocytochemistry analysis of Daudi cells labeling RPA70 with Rabbit anti-RPA70 antibody (ET7107-51) at 1/500 dilution.

Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-RPA70 antibody (ET7107-51) at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
ET7107-51_4.jpg Fig4: Immunocytochemistry analysis of C6 cells labeling RPA70 with Rabbit anti-RPA70 antibody (ET7107-51) at 1/100 dilution.

Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-RPA70 antibody (ET7107-51) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
ET7107-51_5.jpg Fig5: Flow cytometric analysis of Daudi cells labeling RPA70.

Cells were fixed and permeabilized. Then stained with the primary antibody (ET7107-51, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
ET7107-51_6.jpg Fig6: Flow cytometric analysis of C6 cells labeling RPA70.

Cells were fixed and permeabilized. Then stained with the primary antibody (ET7107-51, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
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