RPA70 Recombinant Rabbit Monoclonal Antibody [JB75-32]
cat.: ET7107-51
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IF-Cell, IF-Tissue, FC |
| Clonality: | Monoclonal |
| Clone number: | JB75-32 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 68 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human RPA70 aa 22-71 / 616. |
| Positive control: | Hela cell lysates, A549, LOVO, 293T. |
| Subcellular location: | Nucleus. |
| Recommended Dilutions:
WB IF-Cell FC |
1:500 1:50-1:100 1:50-1:100 |
| Uniprot #: | SwissProt: P27694 Human |
| Alternative names: | Dmrpa1 Drosophila Replication Protein A DRPA HSSB Human single stranded DNA binding protein MST075 MSTP075 p70 REPA1 Replication factor A Replication factor A protein 1 Replication protein A 70 kDa DNA-binding subunit Replication protein A 70kDa DNA binding subunit Replication protein A1 70kDa Replication protein A1 RF A RF-A protein 1 RFA RFA1_HUMAN RP A RP-A p70 RPA 70 RPA rpa1 Single stranded binding protein 70 Single-stranded DNA-binding protein |
Images
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Fig1:
Western blot analysis of RPA70 on Hela cell lysates with Rabbit anti-RPA70 antibody (ET7107-51) at 1/500 dilution. Lysates/proteins at 10 µg/Lane. Predicted band size: 92 kDa Observed band size: 92 kDa Exposure time: 2 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7107-51) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
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Fig2: ICC staining RPA70 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig3: ICC staining RPA70 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig4: ICC staining RPA70 in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig5: Flow cytometric analysis of 293T cells with RPA70 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.