Creatine kinase B type Recombinant Rabbit Monoclonal Antibody [JB78-34]
cat.: ET7107-52
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IF-Cell, IF-Tissue, IHC-P, FC
Clonality: Monoclonal
Clone number: JB78-34
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 43 kDa
Isotype: IgG
Immunogen: Recombinant protein within Human Creatine kinase B type aa 27-118 / 381.
Positive control: HeLa cell lysate, SH-SY5Y cell lysate, Y79 cell lysate, mouse brain tissue lysate, rat brain tissue lysate, HAP1 cell lysate, mouse brain tissue, 293T, LOVO.
Subcellular location: Cytoplasm, cytosol, Mitochondrion, Cell membrane.
Recommended Dilutions:
  WB
  IF-Cell
  IHC-P
  FC
1:2,000
1:50-1:200
1:1,000
1:50-1:100
Uniprot #: SwissProt: P12277 Human | Q04447 Mouse | P07335 Rat
Alternative names: B CK B-CK BB-CK BCK Brain creatine kinase Ckb CKBB Creatine kinase B Creatine kinase B chain Creatine kinase B type Creatine kinase B-type Creatine Kinase BB Isoenzyme Creatine kinase brain Creatine kinase brain type Creatine phosphokinase BB Epididymis luminal protein 211 Epididymis secretory protein Li 29 HEL 211 HEL S 29 KCRB_HUMAN
Images
ET7107-52_1.jpg Fig1: Western blot analysis of Creatine kinase B type on different lysates with Rabbit anti-Creatine kinase B type antibody (ET7107-52) at 1/2,000 dilution.

Lane 1: HeLa cell lysate
Lane 2: SH-SY5Y cell lysate
Lane 3: Y79 cell lysate
Lane 4: Mouse brain tissue lysate
Lane 5: Rat brain tissue lysate

Lysates/proteins at 20 µg/Lane.

Predicted band size: 43 kDa
Observed band size: 43 kDa

Exposure time: 4 seconds; ECL: K1801;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7107-52) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
ET7107-52_2.jpg Fig2: Western blot analysis of Creatine kinase B type on different lysates with Rabbit anti-Creatine kinase B type antibody (ET7107-52) at 1/1,000 dilution.

Lane 1: HAP1-parental cell lysate
Lane 2: HAP1-Creatine kinase B type KD cell lysate

Lysates/proteins at 10 µg/Lane.

Predicted band size: 43 kDa
Observed band size: 43 kDa

Exposure time: 6 seconds; ECL: K1801;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7107-52) at 1/1,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
ET7107-52_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-Creatine kinase B type antibody (ET7107-52) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7107-52) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET7107-52_4.jpg Fig4: ICC staining of Creatine kinase B type in 293T cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET7107-52, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ET7107-52_5.jpg Fig5: ICC staining of Creatine kinase B type in LOVO cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET7107-52, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ET7107-52_6.jpg Fig6: Flow cytometric analysis of Creatine kinase B type was done on 293T cells. The cells were fixed, permeabilized and stained with the primary antibody (ET7107-52, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.